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Molecular and Cellular Neuroscience Course 2016
 
February 15 - 19
Coordinator: Ana Luísa Carvalho (alc@cnc.uc.pt)
PD in Experimental Biology and Biomedicine (PDBEB)
CNC - Center for Neuroscience and Cell Biology, University of Coimbra

Molecular and Cellular Neuroscience Course 2016
February 15-19, 2016
CNC Auditorium

Coordinator:
Ana Luísa Carvalho (alc@cnc.uc.pt)

Lecturers:
Ana Luísa Carvalho, CNC and Dep Life Sciences, UC
Carlos B. Duarte, CNC and Dep Life Sciences, UC
Emília Duarte, CNC and Dep Life Sciences, UC
Inbal Israely, Champalimaud Foudation, Lisbon
João Peça, CNC, UC
Jorge Valero, Achucarro Basque Center for Neuroscience, Spain
Paulo Pinheiro, CNC, UC
Ramiro D. Almeida, CNC, UC
Ricardo Rodrigues, CNC, UC
Rodrigo Cunha, CNC and Faculty of Medicine, UC

COURSE SYLLABUS

February 15
9.00h-9.15h: Structure of the course; Introduction of students and lecturers
9.15h-10.30h: Neurogenesis – Jorge Valero
11.00h-12.30h: Neuronal migration- Ricardo Rodrigues
13.30h-15.00h: Molecular triggers of synapse formation and maturation - Ramiro D. Almeida
15.30h-17.00h: Electrophysiology: a primer (I) - João Peça

February 16
9.00h-10.15h: Electrophysiology: a primer (II) - Paulo Pinheiro
10.45h-12.15h: Presynaptic function - Paulo Pinheiro
14.00h-15.30h: Postsynaptic receptor function - Ana Luisa Carvalho

February 17
9.00h-10.15h: Synaptic plasticity – mechanisms - Ana Luisa Carvalho
10.45h-12.00h: Synaptic plasticity & memory - Ana Luisa Carvalho
14.00h-15.15h: Synaptic modulation by trophic factors– Carlos Duarte
15.45h-17.00h: Neuromodulation by purines – Rodrigo Cunha

February 18
9.00h-10.00h: Glia cells and synaptic function - Emília Duarte
10.30h-12.00h: Imaging neuronal function – Inbal Israely
13.30h-15.00h: Structural plasticity – Inbal Israely

February 19
Morning & Afternoon: Hands-on session on electrophysiology – Paulo Pinheiro


Student Assignment:
The students will write a grant proposal based on the results presented in an original paper. The grant proposal is 3 pages long and should include the following sections: a) State of the art; b) Objectives of proposal; 3) Proposed tasks and methodology. The students will meet with tutors throughout the week to discuss their ideas and approaches for the proposal.
Friday, Fev 26, – Written grant proposal delivery


Background Reading:

Neurogenesis & Neural Patterning:

Ulloa F and Martí E (2010) Wnt Won the War: Antagonistic Role of Wnt over Shh Controls Dorso-Ventral Patterning of the Vertebrate Neural Tube. DEVELOPMENTAL DYNAMICS 239:69–76

Neuronal Migration:

Oscar Marín, Manuel Valiente, Xuecai Ge, et al  (2010) Guiding Neuronal Cell Migrations. Cold Spring Harb Perspect Biol 2010;2:a001834

Synaptogenesis

A. Kimberley McAllister (2007) Dynamic Aspects of CNS Synapse Formation. Ann Rev. Neurosci. 30:425–50

Neural Signaling, Channels and Transporters

Neuroscience, D. Purves et al.

Presynaptic Mechanisms:

Sudhof T (2013) Neurotransmitter Release: The Last Millisecond in the Life of a Synaptic Vesicle., Neuron 80,675.

Synaptic Plasticity:

Huganir RL and Nicoll RA (2013) AMPARs and Synaptic Plasticity: The Last 25 Years. Neuron 80, 704.

Glia & neuronal regulation

Narges Bazargani & David Attwell (2016) Astrocyte calcium signaling: the third wave. Nature Neuroscience; doi:10.1038/nn.4201

Two-Photon Microscopy

Svoboda K and Yasuda R (2006) Principles of Two-Photon Excitation Primer Microscopy and Its Applications to Neuroscience. Neuron 50, 823–839.

 

Papers for Grant proposals:

Skyler L. Jackman, Josef Turecek, Justine E. Belinsky & Wade G. Regehr (2016) The calcium sensor synaptotagmin 7 is required for synaptic facilitation. Nature 529, 88.
Marcos Gomes; contact Paulo Pinheiro (pcspinheiro@gmail.com)

Joana M. Marques, Ricardo J. Rodrigues, Sergio Valbuena, Jose L. Rozas, Sanja Selak, Philippe Marin, Maria I. Aller, and Juan Lerma (2013) CRMP2 Tethers Kainate Receptor Activity to Cytoskeleton Dynamics during Neuronal Maturation. J Neurosci 33(46):18298.
M.ª Inês Martins; contact Ricardo Rodrigues (ricardojrodrigues@gmail.com)

Frank H Yu, Massimo Mantegazza, Ruth E Westenbroek, Carol A Robbins, Franck Kalume, Kimberly A Burton, William J Spain, G Stanley McKnight, Todd Scheuer & William A Catterall (2006) Reduced sodium current in GABAergic interneurons in a mouse model of severe myoclonic epilepsy in infancy. Nature Neurosci, 9: 1142.
Luís Martins; contacto João Peça (jpeca@cnc.uc.pt)

Olde Loohuis NF, Ba W, Stoerchel PH, Kos A, Jager A, Schratt G, Martens GJ, van Bokhoven H, Nadif Kasri N, Aschrafi A (2015) MicroRNA-137 Controls AMPA-Receptor-Mediated Transmission and mGluR-Dependent LTD. Cell Rep. 11(12):1876.
João Amorim; contact Ana Luisa Carvalho (alc@cnc.uc.pt)

Ana Rita Santos, Miranda Mele, Sandra H. Vaz, Blanka Kellermayer, Maddalena Grimaldi, Mariana Colino-Oliveira, Diogo M. Rombo, Diogo Comprido, Ana M. Sebastião, and Carlos B. Duarte (2015) Differential Role of the Proteasome in the Early and Late Phases of BDNF-Induced Facilitation of LTP. J Neurosci 35(8):3319.
Cândida Dias; contact Carlos Duarte (cbduarte@ci.uc.pt)

Kamal Sharma, Se-Young Choi, Yong Zhang, Thomas J.F. Nieland, Shunyou Long, Min Li, and Richard L. Huganir (2013) High-Throughput Genetic Screen for Synaptogenic Factors: Identification of LRP6 as Critical for Excitatory Synapse Development. Cell Rep 5, 1330.
Ana Raquel Coelho; contact Ramiro Almeida (ramirodalmeida@gmail.com)

Yong Zhang, Robert H Cudmore, Da-Ting Lin, David J Linden & Richard L Huganir (2015) Visualization of NMDA receptor–dependent AMPA receptor synaptic plasticity in vivo. Nature Neuroscience 18(3):402.
Daniela Madeira, Miguel Lopes & Miguel Lemos; contact Ana Luisa Carvalho (alc@cnc.uc.pt)

Naiyan Chen, Hiroki Sugihara, Jitendra Sharma, Gertrudis Perea, Jeremy Petravicz, Chuong Le, and Mriganka Sur (2012) Nucleus basalis-enabled stimulus-specific plasticity in the visual cortex is mediated by astrocytes. PNAS 109(41):E2832-41.
Carla Henriques & Ricardo Martins; contact Emília Duarte (epduarte@ci.uc.pt)

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