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Molecular and Cellular Neuroscience Course 2015
February 16 - 27
Ana Luísa Carvalho (alc@cnc.uc.pt)
PD in Experimental Biology and Biomedicine
CNC - Center for Neuroscience and Cell Biology, University of Coimbra

Molecular and Cellular Neuroscience Course 2015
February 16-27, 2015
Caixa Geral Depósitos Room, 1st Floor FMUC

Coordinator: Ana Luísa Carvalho (alc@cnc.uc.pt)

Ana I Oliveira, CNC
Ana Luísa Carvalho, CNC and Dep Life Sciences, UC
Carlos B. Duarte, CNC and Dep Life Sciences, UC
Emília Duarte, CNC and Dep Life Sciences, UC
João Peça, CNC
Jorge Valero, CNC
Paulo Pinheiro, CNC
Ramiro D. Almeida, CNC
Ricardo Rodrigues, CNC


Week 1:

February 16

9.00h-9.15h: Structure of the course; Introduction of students and lecturers
9.15h-10.15h: Neurogenesis - Jorge Valero
10.45h-11.45h: Neuronal migration - Ricardo Rodrigues
13.30h-14.45h: Introduction to synapses: structure-function and molecular composition - Ana Luisa Carvalho

February 17

9.00h-10.15h: Electrophysiology: a primer (I) - João Peça
10.45h-12.00h: Electrophysiology: a primer (II) - Paulo Pinheiro
14.00h-15.30h: Molecular triggers of synapse formation and maturation - Ramiro D. Almeida

February 18

9.00h-10.15h: Presynaptic function - Paulo Pinheiro
10.45h-12.00h: Postsynaptic receptor function - Ana Luisa Carvalho
14.00h-15.30h: Synaptic plasticity & molecular mechanisms - Ana Luisa Carvalho

February 19
9.00h-10.15h: Structural plasticity - Ana Oliveira
10.45h-12.00h: Imaging neuronal function - Ana Oliveira
14.00h-15.30h: Synaptic plasticity and memory - Ana Luisa Carvalho

February 20
9.00h-10.15h: Glia cells and synaptic function - Emília Duarte
10.45h-12. 00h: Synaptic modulation - Carlos B. Duarte

13.30h - Seminar: Sandra Macedo-Ribeiro, IBMC, U Porto

Week 2:

Student Assignment:

The students will individually write and present a grant proposal based on the results presented in an original paper. The grant proposal is 3 pages long and should include the following sections: a) State of the art; b) Objectives of proposal; 3) Proposed tasks and methodology. The students will meet with tutors throughout the week to discuss their ideas and approaches for the proposal.

Thursday, Fev 26, 15h – Written grant proposal delivery

Friday, Feb 27 – Grant proposal presentation and discussion

February 27th - grant proposal presentations:

14:30h: Pedro Curto

15:00h: Helena Pinheiro

15:30h: Mª Inês Veríssimo

16:00h: Laura Oliveira

16:30h: Filipa Almeida

17:00h: Mafalda Costa

17:30h: Vanessa Henriques

18:00h: Mónica Silva

Papers for grant proposals:

1.    Kamal Sharma, Se-Young Choi, Yong Zhang, Thomas J.F. Nieland, Shunyou Long, Min Li, and Richard L. Huganir (2013) High-Throughput Genetic Screen for Synaptogenic Factors: Identification of LRP6 as Critical for Excitatory Synapse Development. Cell Reports 5, 1330–1341.

2.    Joana M. Marques, Ricardo J. Rodrigues, Sergio Valbuena, Jose L. Rozas, Sanja Selak, Philippe Marin, Maria I. Aller, and Juan Lerma (2013) CRMP2 Tethers Kainate Receptor Activity to Cytoskeleton Dynamics during Neuronal Maturation. J Neurosci  33(46):18298 –18310.

3.    Frank H Yu, Massimo Mantegazza, Ruth E Westenbroek, Carol A Robbins, Franck Kalume, Kimberly A Burton, William J Spain, G Stanley McKnight, Todd Scheuer & William A Catterall (2006) Reduced sodium current in GABAergic interneurons in a mouse model of severe myoclonic epilepsy in infancy. Nature Neuroscience 9, 1142.

4.    Paulo S. Pinheiro, Anna M. Jansen, Heidi de Wit, Bassam Tawfik, Kenneth L. Madsen, Matthijs Verhage, Ulrik Gether, and Jakob B. Sørensen (2014) The BAR Domain Protein PICK1 Controls Vesicle Number and Size in Adrenal Chromaffin Cells. J Neurosci 34(32):10688 –10700.

5.    Yong Zhang, Robert H Cudmore, Da-Ting Lin, David J Linden & Richard L Huganir (2015) Visualization of NMDA receptor–dependent AMPA receptor synaptic plasticity in vivo. Nature Neuroscience published online 2 February 2015; doi:10.1038/nn.3936.

6.    Ana Rita Santos, Miranda Mele, Sandra H. Vaz, Blanka Kellermayer, Maddalena Grimaldi, Mariana Colino-Oliveira, Diogo M. Rombo,  Diogo Comprido, Ana M. Sebastião, and Carlos B. Duarte (2015) Differential Role of the Proteasome in the Early and Late Phases of BDNF-Induced Facilitation of LTP. J Neurosci (In Press).

7.    Naiyan Chen, Hiroki Sugihara, Jitendra Sharma, Gertrudis Perea, Jeremy Petravicz, Chuong Le, and Mriganka Sur (2012) Nucleus basalis-enabled stimulus-specific plasticity in the visual cortex is mediated by astrocytes. PNAS 109(41):E2832-41.

Background Reading:

Neurogenesis & Neural Patterning:
Ulloa F and Martí E (2010) Wnt Won the War: Antagonistic Role of Wnt over Shh Controls Dorso-Ventral Patterning of the Vertebrate Neural Tube. DEVELOPMENTAL DYNAMICS 239:69–76
Neuronal Migration:
Oscar Marín, Manuel Valiente, Xuecai Ge, et al  (2010) Guiding Neuronal Cell Migrations. Cold Spring Harb Perspect Biol 2010;2:a001834
A. Kimberley McAllister (2007) Dynamic Aspects of CNS Synapse Formation. Ann Rev. Neurosci. 30:425–50
Neural Signaling, Channels and Transporters
Neuroscience, D. Purves et al.
Presynaptic Mechanisms:
Sudhof T (2013) Neurotransmitter Release: The Last Millisecond in the Life of a Synaptic Vesicle., Neuron 80,675.
Synaptic Plasticity:
Huganir RL and Nicoll RA (2013) AMPARs and Synaptic Plasticity: The Last 25 Years. Neuron 80, 704.
Two-Photon Microscopy
Svoboda K and Yasuda R (2006) Principles of Two-Photon Excitation Primer Microscopy and Its Applications to Neuroscience. Neuron 50, 823–839.


Pedro Curto (pedrocurtobioq@gmail.com)

Sulaiman Abuhaiba (sabuhaiba@gmail.com)

Mafalda Costa (mafaldascosta7@gmail.com)

Filipa Almeida (filipa.almeida1994@gmail.com)

Helena Pinheiro (hellenapinheiroo@gmail.com)

Vanessa Henriques (vanessajh _@hotmail.com)

Yolanda Afonso (yoyoafonso@gmail.com)

Ana R Xavier (anax__@hotmail.com)

Joana Real (cucareal@gmail.com)

Daniela Costa (daniela.ad.costa@gmail.com)

Ana Patrícia Simões (anapatriciafrsimoes@gmail.com)

Ana Sofia Lourenço (anasofiatl@gmail.com)

Laura Oliveira (milena_o@sapo.pt)

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