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CNC Cores
 
September 18 - 21
Microscopy - Luísa Cortes (lcortes@cnc.uc.pt) Flow Cytometry - Isabel Nunes (inunes@cnc.uc.pt) Mass Spectrometry - Bruno Manadas (bmanadas@gmail.com)

CNC Cores Course, September 18 – 21, 2012

Luísa Cortes,  Isabel Nunes Correia, Bruno Manadas

lcortes@cnc.uc.pt; inunes@cnc.uc.pt; bmanadas@gmail.com

Scope: This course aims to provide students with the basic concepts of microscopy, flow cytometry and mass spectrometry, and their applications in research.

3 day course, September 18-20, 2012

Evaluation: written exam (September, 21)

Day 1 - Tuesday, 18 - Luísa Cortes - Microscopy (CNC - BEB room)
Day 2 - Wednesday 19 - Isabel Nunes Correia - Flow Cytometry (CNC - BEB room)
Day 3 - Thursday, 20 - Bruno Manadas - Mass Spectrometry
Friday: 14h - 15h30 - Written Exam (CNC - BEB room)


Day 1 - Tuesday, 18 - Luísa Cortes – Microscopy (CNC - BEB room)

09.30 – Introduction to Fluorescence Microscopy – Luísa Cortes (CNC)
10.30 – Live cell Imaging  - Luísa Cortes (CNC)
11.00 – Coffee break
11.30 – Quantitative imaging approaches to evaluate the synaptic plasticity in cultured neurons - Sandra Santos CNC)
12:00 – Lunch break
14:00 – Mitochondria and autofagosomes intracellular traffic in Parkinsons disease - Sandra Morais Cardoso (CNC, FMUC)
14:30 – Single Cell Calcium Imaging to unravel stem cell-derived phenotypes - João Malva (FMUC, CNC)
15.00 – Coffee break
15:30 – Introduction to Laser Microdissection – Luísa Cortes (CNC)
16.00 – Visit to the Microscopy Unit

PDF1 Laser Microdissection

PDF2 Mic Class 2012

PDF3 Sandra Santos


Day 2 - Wednesday, 19 - Isabel Nunes Correia – Flow cytometry (CNC - BEB room)

09.30 – Introduction to Flow Cytometry, Isabel Nunes Correia (CNC)
11.00 – Coffee break
11.30 – Introduction to Cell Sorting, Isabel Nunes Correia (CNC)
12.00 – Evaluating transduction and silencing of the mouse brain by flow cytometry, Mariana Conceição, (CNC, Faculty of Pharmacy)
12.30 – Lunch break
14.30 – Research and clinical applications of flow cytometry and cell sorting in immunology and oncology, Paulo Rodrigues Santos (Faculty of Medicine, CNC)
15.00 – Study of sperm mitochondrial function through flow cytometry analysis, Sandra Amaral (CNC, Centrum für Reproduktionsmedizin und Andrologie, Universitätsklinikum Münster)
15.30 – FACS as a tool for drug delivery, gene silencing and cell viability assessment, Nuno A. Fonsecaa,b, Sérgio Simõesa,b, João Nuno Moreiraa,b (aCNC, bFaculty of Pharmacy)

PDF1 (Flow Cytometry)

PDF2 (Sorting)

PDF3 (Paulo Santos presentation)


Day 3 - Thursday, 20 - Bruno Manadas – Mass Spectrometry (BIOCANT, Cantanhede)

09.00 – 10.30  Introduction to Proteomics
10.30-10.40     coffee break
10.40-12.30     Introduction to Mass Spectrometry
12.30-13115    Lunch break
13.15-14.00     Quantification by mass spectrometry approaches
14.00-14.10     coffee break
14.10-15.00     Neuroproteomics in practice

 Day 4 - Friday, 21 (CNC - BEB room)

14.00 – 15.30 – Written Exam

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