Doctoral Programme in Experimental Biology and Biomedicine Course “Gene and Cell therapy of CNS: from microRNAs to IPS cells and gene repair” January 7 to 11, 2013
This course will focus on the latest research in the field of gene and cell therapy, including viral and non-viral vectors, in situ and systemic delivery of nucleic acids, gene silencing, microRNAs and induced pluripotent stem cells. Some of the latest achievements in these domains will be presented by local and international experts in the scientific domain.
Monday (Jan 7) Gene Therapy
9:30-10:00- Presentation of the course, students and speakers Luís Pereira de Almeida
10:00-12:00- Gene Therapy – Present and future perspectives Luís Pereira de Almeida and Edgardo Rodriguez
12:00-14:30- Lunch
14:30-15:15- Gene Silencing Edgardo Rodriguez
15:30-16:00- Silencing Machado-Joseph disease in the cerebellum Clévio Nóbrega
16:00-16:30- Gene Delivery to the brain through non-invasive routes Rui Nobre
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Tuesday (Jan 8) MicroRNAs
9:30-10:15 – Gene therapy of lysossomal diseases Edgardo Rodriguez
10:30-11:00 – Non-viral-Gene therapy Liliana Mendonça
11:30-12:30 – miRNAs as master regulators in cell biology and human disease Francisco J. Enguita
12:30-14:30- Lunch
14:30 - 15:15 The dark (and funniest) side of the genome Francisco J. Enguita
15:30 – 16:00 MicroRNAs and microglia Ana Luísa Cardoso 16:00 – 16:30 MicroRNAs and glioblastoma Pedro Costa
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Wednesday (Jan 9) Gene repair
9:30-10:30 - Editing the genome: from zinc fingers to tale nucleases Neville Sanjana
10:30-11:00 – Break 11:00 -12:00 – Seminar: Edgardo Rodriguez Splice isoform-specific suppression of the Cav2.1 variant underlying Spinocerebellar ataxia type 6.
12:00-14:30- Lunch
14:30-15:30 – Using optogenetics to dissect function and disease within the central nervous system João Peça
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Thursday (Jan 10)
9:30-10:15 Reprogrammming fibroblasts into induced pluripotent stem cells and neurons Isabel Onofre
10:30-11:00 – Stem Cells: the case of MSCs Catarina Miranda
11:15-12:15 - Seminar: Using TALENs to model neurological disease in human pluripotent stem cells Neville Sanjana
12:30-14:00- Lunch
14:00-15:00 – Engineering and scaling-up stem cell production Margarida Serra
15:30-17:30- Paper presentations by students
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Friday (Jan 11)
9:30-12:30- Paper presentations by students
17:00-17:20- Multiple choice test
Invited lecturers:
Edgardo Rodriguez Departments of Molecular Physiology and Biophysics, Internal Medicine and Neurology University of Iowa, Iowa City, IA 52242 USA
Neville Sanjana Broad Institute 7 Cambridge Center Cambridge, MA 02142, USA
Francisco Enguita Instituto de Medicina Molecular Faculdade de Medicina, Universidade de Lisboa, Lisboa, Portugal
Margarida Serra IBET- Instituto de Biologia Experimental e Tecnológica Av. República, Qta. do Marquês (EAN) 2784-505 Oeiras- Portugal
CNC
Coordinator: Luís Pereira de Almeida CNC & Faculty of Pharmacy University of Coimbra Coimbra, Portugal
Lecturers:
Clévio Nóbrega CNC University of Coimbra Coimbra, Portugal
Rui Nobre CNC University of Coimbra Coimbra, Portugal
Ana Luísa Cardoso CNC University of Coimbra Coimbra, Portugal
João Peça CNC University of Coimbra Coimbra, Portugal
Pedro Costa CNC & Faculty of Sciences and Technology University of Coimbra Coimbra, Portugal
Liliana Mendonça CNC University of Coimbra Coimbra, Portugal
Isabel Onofre CNC & Faculty of Pharmacy University of Coimbra Coimbra, Portugal
Papers for presentation and discussion:
10:30-12:30- Presentations (max. 20 min/group)
Group 1- Andreia Gomes, Maria do Céu Lourenço, Oksana Trotsenko Paper 1 Group 2 - Dina Pereira, Mohamed Hussien, Cátia Santa, Paper 2
Group 3 - Gladys Caldeira, RaviKumar Kapavarapu, Sandra Anjo Paper 3 Group 4- Ivan Salazar, Sara Oliveira, Inês Santarino Paper 4 Group 5- Jeannette Schmidt, Sofia Ferreira, Elda Bonifácio Paper 5
Group 6- Lara Franco, Ana Cristina Ferreira, Maria Inês Sousa Paper 6
Group 7- Susana Sampaio, Iryna Voytyuk, Helena Antunes Paper 7
Group 8- Patrícia Morais, Eskedar Angamo, Josephine Blersch Paper 8
Group 9- Vitor Carmona, Sara Silva, Geetha, Patricia Albuquerque Paper 9
17:00-17:20- Multiple-choice examination
Students Assignments and Distribution
The assignments will include:
- Participation during the course
- Paper presentation & fictive projects (paper presentation, critical review of paper, proposal novelty, hypothesis-driven proposal, expected results) - Multiple choice examination
Students are asked to consider an assigned paper as representing a pivotal work, briefly present it and prepare a brief project stating what experiments would be important to do next.
While briefly mentioning the paper findings, the students should concentrate on which directions the research would take. Apart from presenting data selected from the paper, presentation of the project should have the following points:
-Title
- State of the Art (include some data from the assigned paper)
-Objectives
- Tasks (maximum 3, including expected results and descriptions)
The fictive projects will be presented (20 min) and discussed (10 min) during Thursday afternoon and Friday morning (10:30-12:30)
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