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Biomedical Magnetic Resonance: Molecular Imaging & Metabolism
 
January 4 - 6
Carlos Geraldes (geraldes@bioq.uc.pt), John Jones
CENTER FOR NEUROSCIENCE AND CELL BIOLOGY (CNC)
Ph. D. Program in Experimental Biology and Biomedicine (BEB)

PhD. Course: BIOMEDICAL MAGNETIC RESONANCE:
MOLECULAR IMAGING AND METABOLISM

Area:     New Techniques in Biomedicine

Duration:     4-6 January 2012

Location:     Anfiteatro 2.º andar - CNC, Edifício FMUC

Coordinators:     Carlos Geraldes(geraldes@ci.uc.pt), John Jones (jones@cnc.cj.uc.pt) (CNC, Coimbra)

Lecturers:
    
Arend Heerschap    Department of Radiology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands                         

Robert Muller    Department of General, Organic and Biomedical Chemistry and Faculty of Medicine and Pharmacy, University of Mons, Belgium.
 
Objectives and Scope:  The objectives of this course are to introduce the Ph.D. students to current important applications of Nuclear Magnetic Resonance (NMR) in Biomedicine, both in spectroscopy and imaging. These are very dynamic and exciting research fields, where new developments are expanding the applicability of Biomedical NMR for noninvasive analyses of animal disease models and humans. The topics to be covered are: a) application of the NMR methodology with the use of stable isotope tracers (e.g. 13C, 2H, 15N) to metabolic studies, from basic to clinical applications in health and disease,  b) In vivo 1H and 13C MRS studies of human physiology pathologies and c) MRI-based and multimodal molecular imaging studies, including magnetic cell labeling and tracking, which are currently of very high interest; the use of Nanosciences (eg. liposomes, nanoparticles) in the development of contrast agents for Molecular Imaging and image-guided therapy using MRI and multimodal approaches.
This course will be included in the programmed proposed activities of the National NMR Network, to which the CNC participates through the NMR Laboratory.

Organization of the course: There will be lectures, with an informal format, and Journal Club-like Discussions. All students will have access to representative papers published by the Faculty Members and are strongly encouraged to read them beforehand. This Course in designed for Ph.D. students following the CNC Program in Experimental Biology and Biomedicine (BEB), but is also open to all other students, members of the National NMR Network and the general public.
BEB students (and other Ph.D. students who wish to participate for credit) will be asked to present assigned papers (i.e., PowerPoint presentations). Assignments will be discussed by the Invited Lecturers according to the program and these will form a significant part of each student´s evaluation.
PhD students that are not in the BEB Program are welcome to participate in this course for credit. In order to be admitted they must register for the Course with the organizer two weeks before the Course starts (i.e. 21 December 2011) so that the discussion papers can be assigned to them.


Course Program


Wednesday, January 4: In vivo 13C NMR and Metabolism

9.30 -11.00 h:     In vivo 13C NMR studies of metabolism: History and Introductory concepts
Arend Heerschap

11.30-13.00 h:     Applications of in vivo 13C NMR spectroscopy to human metabolic studies
Arend Heerschap
        
15.00-17.30 h:     Analysis and discussion of papers by the students


Thursday, January 5: MRI and Molecular Imaging


19.30 -11.00 h:       “Contrast Agents for Magnetic Resonance Imaging”
    Robert Muller


11.30-13.00 h:     “Tracers for Magnetic Resonance Molecular Imaging”
    Robert Muller

15.00-17.30 h:     Analysis and discussion of papers by the students


Friday, January 6:

10.00-12.00 h:               Free


16.00-17.30 h: CNC Seminar
"Magnetic Resonance Molecular Imaging"
Prof. Robert N. Muller        
Department of General, Organic and Biomedical Chemistry and Faculty of Medicine and Pharmacy,
University of Mons,
Belgium.

Invited Lecturers
                 
1.  Professor Arend Heerschap        
Department of Radiology
Radboud University Nijmegen Medical Centre,
Nijmegen, The Netherlands                         
E-mail: A.Heerschap@rad.umcn.nl

Papers for evaluation:
1) Gated dynamic 31P MRS shows reduced contractile phosphocreatine breakdown in mice deficient in cytosolic creatine kinase and adenylate kinase
Hermien E. Kan, Andor Veltien, Henk Arnts, Christine I. H. C. Nabuurs, Bart Luijtea, Arnold de Haan, Bé Wieringa and Arend Heerschap
NMR Biomed. (2009), (www.interscience.wiley.com) DOI:10.1002/nbm.1364

2) 31P Saturation Transfer Spectroscopy Predicts Differential Intracellular Macromolecular Association of ATP and ADP in Skeletal Muscle
Christine Nabuurs, Bertolt Huijbregts, Be´ Wieringa, Cees W. Hilbers, and Arend Heerschap
J. Biol. Chem., 285, 39588–39596 (2010).
DOI 10.1074/jbc.M110.164665

3) Effect of Acute Hypoglycemia on Human Cerebral Glucose Metabolism Measured by 13C Magnetic Resonance Spectroscopy
Kim C.C. van de Ven, Bastiaan E. de Galan, Marinette van der Graaf, Alexander A. Shestov, Pierre-Gilles Henry, Cees J.J. Tack and Arend Heerschap
Diabetes, 60, 1467-1473, 2011

2. Professor Robert N. Muller        
Department of General, Organic
and Biomedical Chemistry and
Dean of Faculty of Medicine and Pharmacy
University of Mons, Belgium.
E-mail: robert.n.h.muller@gmail.com

Papers for evaluation:
1) “Magnetic labeling of non-phagocytic adherent cells with iron oxide nanoparticles: a comprehensive study”, Sébastien Boutry, Stéphanie Brunin, Isabelle Mahieu, Sophie Laurent,
Luce Vander Elst and Robert N. Muller
Contrast Media Mol. Imaging 2008, 3, 223–232.

2) “Peptidic Targeting of Phosphatidylserine for the MRI Detection of Apoptosis in Atherosclerotic Plaques”, Carmen Burtea, Sophie Laurent, Eric Lancelot, Sébastien Ballet, Oltea Murariu, Olivier Rousseaux, Marc Port, Luce Vander Elst, Claire Corot, and Robert N. Muller
Molecular Pharmaceutics, 2009, 6, 903–1919.

3) “Potential amyloid plaque-specific peptides for the diagnosis of Alzheimer’s disease”
Lionel Larbanoix, Carmen Burtea, Sophie Laurent, Fred Van Leuven, Gérard Toubeau, Luce Vander Elst, Robert N. Muller
Neurobiology of Aging 2010, 31, 1679–1689.

INFO_Professors

Assigments of papers to the students:

1) Gated dynamic 31P MRS shows reduced contractile phosphocreatine breakdown in mice deficient in cytosolic creatine kinase and adenylate kinase
Hermien E. Kan, Andor Veltien, Henk Arnts, Christine I. H. C. Nabuurs, Bart Luijtea, Arnold de Haan, Bé Wieringa and Arend Heerschap
NMR Biomed. (2009), (www.interscience.wiley.com) DOI:10.1002/nbm.1364

Ana Patrícia Marques
Sofia Constantino Romano

2) 31P Saturation Transfer Spectroscopy Predicts Differential Intracellular Macromolecular Association of ATP and ADP in Skeletal Muscle
Christine Nabuurs, Bertolt Huijbregts, Be´ Wieringa, Cees W. Hilbers, and Arend Heerschap
J. Biol. Chem., 285, 39588–39596 (2010).
DOI 10.1074/jbc.M110.164665

André Filipe Soares
Sara Monteiro Lopes
Otília Almeida

3) Effect of Acute Hypoglycemia on Human Cerebral Glucose Metabolism Measured by 13C Magnetic Resonance Spectroscopy
Kim C.C. van de Ven, Bastiaan E. de Galan, Marinette van der Graaf, Alexander A. Shestov, Pierre-Gilles Henry, Cees J.J. Tack and Arend Heerschap
Diabetes, 60, 1467-1473, 2011

António Carvalho Silva
Tânia Marisa Perestrelo


4) “Magnetic labeling of non-phagocytic adherent cells with iron oxide nanoparticles: a comprehensive study”, Sébastien Boutry, Stéphanie Brunin, Isabelle Mahieu, Sophie Laurent,
Luce Vander Elst and Robert N. Muller
Contrast Media Mol. Imaging 2008, 3, 223–232.

Catarina Praça Almeida
Mariline Mendes Silva

5) “Peptidic Targeting of Phosphatidylserine for the MRI Detection of Apoptosis in Atherosclerotic Plaques”, Carmen Burtea, Sophie Laurent, Eric Lancelot, Sébastien Ballet, Oltea Murariu, Olivier Rousseaux, Marc Port, Luce Vander Elst, Claire Corot, and Robert N. Muller
Molecular Pharmaceutics, 2009, 6, 903–1919.

Dominique Moreira Fernandes
Marcelo José M. Correia
Miguel Maia Lino

6) “Potential amyloid plaque-specific peptides for the diagnosis of Alzheimer s disease”
Lionel Larbanoix, Carmen Burtea, Sophie Laurent, Fred Van Leuven, Gérard Toubeau, Luce Vander Elst, Robert N. Muller
Neurobiology of Aging 2010, 31, 1679–1689.

João Ribas Almeida
Joana Ribeiro Guedes
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