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CNC Cores (Mass Spectrometry, Flow Cytometry and Microscopy)
 
September 20 - 23
Bruno Manadas, Isabel Nunes Correia, Luísa Cortes

bmanadas@gmail.com, inunes@cnc.uc.pt, lcortes@cnc.uc.pt
CNC Cores Course

Bruno Manadas, Isabel Nunes Correia, Luísa Cortes
bmanadas@gmail.com; inunes@cnc.uc.pt; lcortes@cnc.uc.pt

Scope: This course aims to provide the students with the basic concepts of microscopy, flow cytometry and mass spectrometry, and their applications in research.


3 day course, September 20-22, 2011


Evaluation: written exam (September, 23)


Day 1 – Tuesday, 20 - Luísa Cortes – Microscopy (CNC – BEB room)

Day 2 – Wednesday, 21 - Isabel Nunes Correia – Flow Cytometry (CNC – BEB room)

Day 3 – Thursday, 22 - Bruno Manadas – Mass Spectrometry (Biocant)

Friday: 14h – 15h30 - Written Exam (CNC – BEB room)


Day 1 – Tuesday, 20 - Luísa Cortes – Microscopy (CNC – BEB room)
 
9.30   - 11.00 Principles of Fluorescence Microscopy and Live Cell Imaging Techniques. Luísa Cortes (CNC)
11.00 - 11.30  Coffee break
11.30 - 12.30 Quantitative imaging approaches to evaluate the synaptic expression of glutamate receptors in culture neurons, Sandra Santos (CNC)
12.00 - 14.30  Coffee break
14.30 - 15.00 Mitochondrial metabolism dictates the autophagic outcome, Sandra Cardoso (CNC, FMUC)
15.00 - 15.30 To be announced, João Malva (CNC, FMUC)
15.30 - 16.00  Coffee break
16.00 - 17.00 Principles and Applications of Laser Microdissection. Luísa Cortes (CNC)

aula mic BEB 2011.pdf

confocal mic in living cells.pdf

confocal mic overview.pdf

Laser capture (nmet2006).pdf

Laser Microdissection BEB 2011

Optical Sectioning_NatureMeth2005

review in live-cell imaging2008.pdf



Day 2 – Wednesday, 21 - Isabel Nunes Correia – Flow Cytometry (CNC – BEB room)

  9.30 - 11.00 Introduction to Flow Cytometry, Isabel Nunes Correia (CNC)
11.00 - 11.30 Coffee break
11.30 - 12.00 Introduction to Sorting, Magno Garcia (CNC)
12.00 - 12.30 Cell Cycle Analysis and Sensitivity of Osteosarcoma Stem Cells to Therapy: a Flow Cytometry Approach, Sara Raquel Martins Neves (IBILI)
12.30 - 14.00 Lunch break
14.00 -14.30 Research and clinical applications of flow cytometry and cell sorting in immunology and oncology, Paulo Rodrigues Santos (FMUC/CNC)
14.30 - 15.00 Drug Delivery, Gene Silencing and Viability Impact Assessment by Flow Cytometry, Nuno André Carvalho da Fonseca (CNC)
15.00 - 15.30 Human sperm populations and subpopulations characterization: implications for fertility, Ana Paula Sousa (CNC, HUC)

Introduction_to_Flow_Cytometry.pdf

Cell Sorting Sept2011.pdf


Day 3 – Thursday, 22 - Bruno Manadas – Mass Spectrometry (Biocant)


10.00 - 11.00 Introduction to Proteomics, Bruno Manadas (CNC)
11.00 - 11.30 Coffee break
11.30 - 12.30 Introduction to Mass Spectrometry, Bruno Manadas (CNC)
12.30 - 14.00 Lunch break
14.00 - 15.30 Quantification by mass spectrometry approaches, Vera Mendes (CNC), Bruno Manadas (CNC)
15.30 - 15.45 Coffee break
15.45 - 16.45 Neuroproteomics in practice, Bruno Manadas (CNC)

Day 4 – Friday, 24 - (CNC – BEB room)

14.00 – 15h30 - Written Exam
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