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Cores CNC
 
October 25 - 29
Responsible: Bruno Manadas (Mass Spectrometry); Carlos Geraldes (RMN); Isabel Nunes-Correia (Flow Cytometry); Luisa Cortes (Miscroscopy)
CNC Cores, October 25th – 28 th, 2010


25th October – RMN (Carlos Geraldes)

11:00 – Introduction and some applications of NMR Spectroscopy – Carlos Geraldes (FCTUC-CNC)
12:00 – Lunch break
14:00 – How to access the NMR facility – Emeric Wasielewsky  (CNC)
14:30 – Protein-ligand interactions by NMR – David Dias (CNC)
15.00 – Coffee break
15:30 – NMR detection of stable isotopes in metabolic tracer studies – John Jones (CNC)
16.00 – Tissue and biopsy characterization by NMR and MRI - Margarida Castro (FCTUC-CNC)


26th October – Microscopy (Luísa Cortes)


09.30 – Introduction to Fluorescence Microscopy – Luísa Cortes (CNC)
10.30 – Fluorescence microscopy as a tool to study synapse formation – Ramiro Almeida (CNC)
11.00 – Coffee break
11.30 – Single Cell Calcium Imaging  - João Malva (FMUC - CNC)
12:00 – Lunch break
14:00 – Localization and co-localization of receptors in nerve terminals – Rodrigo Cunha (FMUC – CNC)
14:30 – Using laser scanning microscopy for co-localization studies in tissue sections and monolayer cultures – Inês Araujo (CNC)
15.00 – Coffee break
15:30 – Introduction to Laser Microdissection – Luísa Cortes (CNC)
16.00 – Laser Microdissetion in Cancer Research– Maria Reis Silva (FMUC)


27th October – Flow Cytometry (Isabel Nunes Correia)


09.30 – Introduction to Flow Cytometry - Isabel Nunes Correia (CNC)
10.45 – Coffee break
11.15 – Cell cycle analysis and sensitivity of tumor cells to therapy: a flow cytometry approach  - Anália do Carmo (CNC)
12:00 – Lunch break
14:00 – Detection of cell proliferation by incorporation of thymidine analogues and cell cycle analysis by flow cytometry in neuronal stem cells -  Inês Morte, Bruno Carreira, Anália do Carmo, Isabel Nunes-Correia, Caetana Carvalho, and Inês Araujo (speaker) 
14.30 – Use of flow cytometry in the development of non-viral vectors for anti-tumor therapy - Sara Trabulo (CNC)
15.00 – Coffee break
15:30 – Margarida Carneiro (CNC)



28th October: Mass Spectrometry and Proteomics

(Bruno Manadas and Vera Mendes)

This day course will be at Biocant Park, Cantanhede

10.00 - 11.00    Introduction to Proteomics – Bruno Manadas (CNC)
11.00 - 11.30    coffee break
11.30 - 12.30    Introduction to Mass Spectrometry – Bruno Manadas (CNC)
12.30 - 14:00    Lunch break
14.00 -15.30    Quantification by mass spectrometry approaches – Vera Mendes (CNC), Bruno Manadas (CNC)
15.30 - 15.45    coffee break
15.45 - 16.45    Neuroproteomics in practice – Bruno Manadas (CNC)
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