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CNC Cores
 
November 3 - 5
Isabel Nunes, Luisa Cortes, Bruno Manadas; John Jones, Carlos Geraldes
Programme

3rd November – Flow Cytometry (Isabel Nunes Correia)
09.30 - Introduction to Flow Cytometry - Isabel Nunes Correia (CNC)
10.30 - Coffee break
11.00 - Against Homogeneity: Characterization of functionally distinct sub-populations of cells using flow cytometry - João Ramalho-Santos (CNC/FCTUC)
12:00 - Lunch break
14:00 - Evaluation of gene silencing strategies by flow cytometry - Lígia C. Gomes
            da Silva (CNC)
15.00 - Coffee break
15:30 - Applications of flow cytometry to cell biology - Helena M. Lourenço Carvalheiro
          (CNC)
 
4th November: Microscopy (Luísa Cortes)
09.30 - Introduction to Fluorescence Microscopy – Luísa Cortes (CNC)
10.30 - Coffee break
11.00 - Membrane traffic in infection and atherosclerosis - Otília Vieira (CNC)
12:00 - Lunch break
14:00 - Functional identification of phenotypes differentiating from neural stem cell cultures - João Malva (CNC/FMUC)
15.00 - Coffee break
15:30 - Principles of Laser Microdissection - Luísa Cortes (CNC)
16:00 - Laser Microdissection: profiling expression of microRNAs - Maria Silva (IAP-FMUC)     
 
5th November: Mass Spectrometry and Proteomics (Bruno Manadas) - This day will be place at Biocant Park, Cantanhede
10.00-11.00 - Introduction to Proteomics – Bruno Manadas (CNC)
11.00-11.30 - coffee break
11.30-12.30 - Introduction to Mass Spectrometry – Bruno Manadas (CNC)
12.30-14:00 - Lunch break
14.00-15.00 - “Detection of novel cancer-associated glycoprotein biomarkers by MALDI-TOF/TOF mass spectrometry” – Hugo Osório (IPATIMUP)
15.00-15.30  - coffee break
15.30-16.30  - Neuroproteomics in practice – Bruno Manadas (CNC)

6th November: Biomedical NMR (Carlos Geraldes and John Jones)
10.00-11.00 - Biomedical NMR, MRI and contrast agents for Molecular Imaging:
                    some applications (Carlos Geraldes)
11.00-11.30 - coffee break
11.30-12.30 - Use of  stable isotopes (13C and 2H) and NMR in metabolic studies
                    (John Jones)
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