Lecturers Carlos F. Geraldes, Ph.D. Department of Biochemistry, FCT, University of Coimbra Sebastián Cerdán, Ph.D. Instituto de Investigaciones Biomédicas “Alberto Sols”, CSIC/Univ. Autonoma de Madrid, Madrid, Spain Craig R. Malloy, M.D. University of Texas Southwestern Medical Center at Dallas and Dallas VA Medical Center, Dallas, Texas, USA Silvio Aime, Ph.D. Chemistry Department and Center for Molecular Imaging, University of Torino, Torino, Italy

Course Schedule Tuesday 1 March: Introduction and Background 9-10h Introduction to Biomedical NMR Spectroscopy   Carlos F. Geraldes 10.30-11.30h Basic NMR Parameters and Their Biomedical Information Content   Carlos F. Geraldes 14-15h Introduction to Magnetic Resonance Imaging (MRI)   Carlos F. Geraldes Wednesday 2 March: 13C and 2H NMR of Brain Metabolism: cells and in vivo studies 9-10h Cellular and subcellular metabolism of water as detected by multinuclear NMR spectroscopy   Sebastián Cerdán 12-13h Energetics of glutamatergic and gabaergic neurotransmissions in the adult mammalian brain   Sebastián Cerdán 14.30-17.30h Analysis and discussion of papers   Hydrogen turnover and subcellular compartmentation of hepatic (2- 13C) glutamate and (3- 13C) aspartate as detected by 13C NMR. García-Martín et al (2002) J. Biol. Chem. 277 ,7799-7807   Ana Filipa Simões   Cerebral glucose metabolism and the glutamine cycle as detected by in vivo and in vitro 13C NMR spectroscopy. García-Espinosa et al (2004) Neurochem. Internat. 45 , 297-303   Susana Isabel Silva   Impaired tricarboxylic acid cycle activity in mouse livers lacking cytosolic phosphoenolpyruvate carboxykinase. Burgess et al (2004) J. Biol. Chem. 279 (47):48941-9   Luís Paulo Valente Thursday 3 March: 13C and 2H NMR of Heart and Liver Metabolism 9-10h Substrate Oxidation in the Heart by 13C NMR   Craig Malloy 10.30-11.30h 2 H and 13C NMR for Liver Metabolism and Clinical Applications   Craig Malloy 14-17h Analysis and discussion of papers   Glucose production pathways by 2H and 13C NMR in patients with HIV-associated lipoatrophy. Weis et al (2004) Magn. Reson. Med., 51 (4):649-54.   Differing Mechanisms of Hepatic Glucose Overproduction in Triiodothyronine-Treated Rats versus Zucker Diabetic Fatty Rats by NMR Analysis of Plasma Glucose. Jin et al, American Journal of Physiology, in press   Ana Margarida Sousa and Bruno Miguel Gago   Improved route for the visualization of stem cells labeled with a Gd-/Eu-chelate as dual (MRI and fluorescence ) agent. S. Geninatti Crich et al. (2004) Magn. Reson. Med., 51, 938-944   Carla Alexandra Bento and João Henrique Duarte   Magnetic resonance imaging visualization of targeted cells by the internalization of supramolecular adducts formed between avidin and biotinylated Gd 3+ chelates. S. Geninatti Crich et al. (2005) J. Biol. Inorg. Chem., 10 , 78-86.   Rita Mafalda Rocha Friday 4 March: Molecular Imaging by NMR 9-10h Basics of MRI Contrast Agents   Silvio Aime 10.30-11.30h Responsive and Targeting MRI Contrast Agents   Silvio Aime Seminars:   15.00 h Fluxes in Metabolic Networks of the Liver by NMR   Craig R. Malloy 16.00 h Magnetic Resonance Contrast Agents for Molecular Imaging Applications   Silvio Aime