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Molecular Cell Biology
 
October 11 - 15
Coordinator: Carlos Duarte

Lecturers
Andras Kapus (Toronto General Hospital Research Institute, Toronto, ON, Canada)
James W.D. Booth (Sunnybrook and Women’s College Research Institute, Toronto, ON, Canada)
Marc G. Coppolino (Department of Microbiology, University of Guelph, Guelph, ON, Canada)
 
COURSE SCHEDULE


Monday (Oct 11)
9:00-10:30  Techniques in Cell Biology
                  A. Kapus, J. Booth & M. Coppolino
11:00-12:00  Overview of endocytosis
                     J. Booth
 
14:00-15:30  Intracellular vesicular transport I
                   M. Coppolino
16:00-17:00  Overview of cell volume regulation
                   A. Kapus


Tuesday (Oct 12)
9:00-10:30  Intracellular vesicular transport II
                   M. Coppolino
11:00-12:00  Osmosensitive channels, carriers and pumps
                   A. Kapus
 
14:00-17:00  Journal Club (M. Coppolino)
                     Reading assignments:
Prigozhina NL, Waterman-Storer CM (2004) Protein kinase D-mediated anterograde membrane trafficking is required for fibroblast motility. Curr Biol. 14: 88–98.  Pdf   (Ana Filipa Simões & Ana Oliveira).
Thakur P, Stevens DR, Sheng Z-H, Rettig J (2004) Effects of PKA-mediated phosphorylation of snapin on synaptic transmission in cultured hippocampal neurons. J Neurosci. 24: 6476–6481Pdf
Kasap M, Thomas S, Danaher E, Holton V, Jiang S, Storrie B (2004) Dynamic nucleation of Golgi apparatus assembly from the endoplasmic reticulum in interphase HeLa cells. Traffic 5: 595-605.   Pdf
(Ana Margarida Sousa & Bruno Gago)


Wednesday  (Oct 13)
9:00-10:30  Current issues in endocytosis
                  J Booth
11:00-12:00  Osmotic signaling pathways and cytoskeletal remodeling
                   A. Kapus
 
14:00-17:00  Journal Club (A. Kapus)


Reading assignments:

Wu KL, Khan S, Lakhe-Reddy S, Jarad G, Mukherjee A, Obejero-Paz CA, Konieczkowski M, Sedor JR, Schelling JR. (2004) The NHE1 Na+/H+ exchanger recruits ezrin/radixin/moesin proteins to regulate Akt-dependent cell survival. J Biol Chem. 279: 26280-26286.  Pdf
(Carla Bento & Luís Valente)
 
Fischer OM, Hart S, Gschwind A, Prenzel N, Ullrich A. (2004) Oxidative and osmotic stress signaling in tumor cells is mediated by ADAM proteases and heparin-binding epidermal growth factor. Mol Cell Biol. 24: 5172-5183. Pdf
(João Lucas Duarte & Rita Rocha)  
 
Go WY, Liu X, Roti MA, Liu F, Ho SN. (2004) NFAT5/TonEBP mutant mice define osmotic stress as a critical feature of the lymphoid microenvironment. Proc Natl Acad Sci U S A. 101: 10673-10678.   Pdf
(Susana Silva)
 
Uhlik MT, Abell AN, Johnson NL, Sun W, Cuevas BD, Lobel-Rice KE, Horne EA, Dell´Acqua ML, Johnson GL. (2003) Rac-MEKK3-MKK3 scaffolding for p38 MAPK activation during hyperosmotic shock. Nat Cell Biol. 5: 1104-1110. (Option)   Pdf


Thursday  (Oct 14)
9:00-10:30  Lipid rafts
                  J. Booth 
11:00-12:00  SNARE-mediated membrane traffic
                   M. Coppolino
 
14:00-17:00  Journal Club (J. Booth)


Reading assignments:  
 
Motley A, Bright NA, Seaman MN, Robinson MS (2003) Clathrin-mediated endocytosis in AP-2-depleted cells. J Cell Biol. 162: 909-918.   Pdf
(João Peça & Rosália Sá)

Puertollano R, Bonifacino JS (2004) Interactions of GGA3 with the ubiquitin sorting machinery. Nat Cell Biol. 6: 244-251.   Pdf
(Carlos Melo & Sofia Fertuzinhos) 
 
Wilson BS, Steinberg SL, Liederman K, Pfeiffer JR, Surviladze Z, Zhang J, Samelson LE, Yang LH, Kotula PG, Oliver JM (2004) Markers for detergent-resistant lipid rafts occupy distinct and dynamic domains in native membranes. Mol Biol Cell. 15: 2580-2592.   Pdf


Friday  (Oct 15)
9:00-10:30  Regulated exocytosis
                  M. Coppolino
11:00-12:00  Osmotic regulation of gene transcription
                   A. Kapus
 
16:00  Seminar: Phosphorylation-independent ubiquitylation and endocytosis of the FcgRIIA receptor
                       
James Booth

 
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