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Cancer Gene Therapy
 
March 14 - 17
Coordinators: Conceição Pedroso-Lima, Sérgio Simões, João Nuno Moreira

Lecturers:
 
 
Esther Chang
 
Lombardi Comprehensive Cancer Center, Georgetown University, Washington  DC
 
Mirco Ponzoni
 
Differentiation Therapy Unit, Laboratory of Oncology, G. Gaslini Children´s Hospital, Genova, Italy
Coordinators:
 
 
 
Center for Neuroscience and Cell Biology, University of Coimbra
 
 
Center for Neuroscience and Cell Biology, University of Coimbra
 
 
Center for Neuroscience and Cell Biology, University of Coimbra


Objectives of the course
The aim of this course is to provide timely, comprehensive and critical surveys, to discuss recent progress and future directions in the field of cancer gene therapy.  Particular emphasis will be devoted to the molecular targets and strategies underlying the different gene therapy approaches against cancer, currently under investigation. Advantages and limitations of these approaches will be discussed. Moreover, the design of target specific and efficient gene delivery vehicles, as a crucial issue for the successful application of gene therapy, will be highlighted. The course also aims to provide the students the opportunity to discuss theoretical and experimental topics with recognized experts in the field.


COURSE SCHEDULE:
 
14th  (Tuesday)
9:30-10:30
Viral and Nonviral Gene Therapy for Cancer
 
E. Chang
Coffee break
 
11:00-12:00
Tumor-targeted Nanodelivery System
 
E. Chang
 
 
14:30-17:00
Presentation (15 min) and discussion of research papers
 
Group I
 
Group II
 
15th  (Wednesday)
9:30-10:30
Targeted delivery of antisense oligonucleotides in cancer, two different mechanisms of action:
 
a) c-myc and melanoma
 
M.Ponzoni
Coffee break
 
11:00-12:00
Targeted delivery of antisense oligonucleotides in cancer, two different mechanisms of action:
 
 b) c-myb and neuroblastoma
 
M.Ponzoni
 
 
14:30-17:00
Presentation (15 min) and discussion of research papers
 
Group III
 
Group IV
 
16th  (Thursday)
11:00-12:00
Tumor targeting as a new therapeutic strategy for neuroectoderma-derived tumors.
 
M.Ponzoni
 
 
14:30-17:00
Presentation (15 min) and discussion of research papers
 
Group V
 
Group VI
 
17th  (Friday)
16:00
Seminar
 
Tumor vascular technology: a new therapeutic strategy for cancer
 
 
Research Papers


Publications relevant for the course
 
C. Fonseca, J.N. Moreira, C.J. Ciudad, M.C. Pedroso de Lima, S. Simões (2005) Targeting of sterically stabilised pH-sensitive liposomes to human T-leukaemia cells. Eur J Pharma Biopharma, 59, 359-366.
 
S. Simões, A. Filipe, H. Faneca, M. Mano, N. Penacho, N. Düzgünes and M.C. Pedroso de Lima (2005). Cationic liposomes for gene delivery. Expert Opin Drug Deliv, 2, 237-254.
 
H. Faneca, S. Simões and M.C. Pedroso de Lima (2004) Association of albumin or protamine to lipoplexes: enhancement of transfection and resistance to serum. Journal of Gene Medicine, 6, 681-692.
 
M.C. Pedroso de Lima, S. Neves, A. Filipe, N. Düzgünes and S. Simões (2003) Strategies to enhance transfection mediated by lipoplexes: From biophysics to therapeutics. Current Medicinal Chemistry, 10, 919-927.
 
Moreira JN, Gaspar R, Allen TM. Targeting Stealth liposomes in a murine model of human small cell lung cancer. Biochimica Biophysica Acta. 2001 Dec 1;1515(2):167-76.
 
Brignole C, Pastorino F, Marimpietri D, Pagnan G, Pistorio A, Allen TM, Pistoia V, Ponzoni M. Immune cell-mediated antitumor activities of GD2-targeted liposomal c-myb antisense oligonucleotides containing CpG motifs. Journal of the National Cancer Institute 2004 Aug 4;96(15):1171-80.
 
Pastorino F, Brignole C, Marimpietri D, Pagnan G, Morando A, Ribatti D, Semple SC, Gambini C, Allen TM, Ponzoni M. Targeted liposomal c-myc antisense oligodeoxynucleotides induce apoptosis and inhibit tumor growth and metastases in human melanoma models. Clinical Cancer Research 2003 Oct 1;9(12):4595-605.
 
Pagnan G, Stuart DD, Pastorino F, Raffaghello L, Montaldo PG, Allen TM, Calabretta B, Ponzoni M. Delivery of c-myb antisense oligodeoxynucleotides to human neuroblastoma cells via disialoganglioside GD(2)-targeted immunoliposomes: antitumor effects.  Journal of the National Cancer Institute 2000 Feb 2;92(3):253-61.
 
Pastorino F, Brignole C, Marimpietri D, Sapra P, Moase EH, Allen TM, Ponzoni M.  Doxorubicin-loaded Fab´ fragments of anti-disialoganglioside immunoliposomes selectively inhibit the growth and dissemination of human neuroblastoma in nude mice.  Cancer Res. 2003 Jan 1;63(1):86-92.
 
Pastorino F, Brignole C, Marimpietri D, Cilli M, Gambini C, Ribatti D, Longhi R, Allen TM, Corti A, Ponzoni M. Vascular Damage and Anti-angiogenic Effects of Tumor Vessel-Targeted Liposomal Chemotherapy. Cancer Res. 2003 November 1,63:7400-7409.

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