About BEB
CNC Research
BEB in the Media
October 24 - 28
Coordinators: Milton Costa and Nuno Empadinhas

Life is everywhere, even in the most extreme environments that one can imagine. How can organisms survive and be metabolically active in the extremely cold environments from Antarctida with subfreezing temperatures? How do microbial life forms thrive on toxic levels of sulfides and heavy metals above magma plumes with extremely high temperatures? How is it possible that organisms exist in the complete absence of light at Km depths in the ocean, buried deep within land masses or in the hot, UV-exposed soils from extremely arid deserts?
The secrets of Life in Extreme Environments are only now beginning to be unravelled and this course provides a general insight into the subject. Extant prokaryotes exhibit an extraordinary range of metabolic versatility that continues to expand as new species are isolated from extreme environments. The course will cover the current knowledge of prokaryotic biodiversity in some of these environments, and explore some of the strategies used by those microorganisms to endure and adapt to such conditions.
The biotechnological impact and the biomedical potential of extremophiles and their products will also be addressed.

Tuesday (Oct 25)
Biodiversity of Prokaryotes
Milton Costa
Meet the Extremophiles! We´re not so tough after all!!
Milton Costa
Wednesday (Oct 26)
Aula 3 (1-PDF ; 2-PDF)
Role and diversity of compatible solutes
Milton Costa
Biosynthesis of compatible solutes in Extremophiles
Nuno Empadinhas
Thursday (Oct 27)
The role of compatible solutes in osmotic and thermoadaptation of Rhodothermus marinus: Biosynthesis and regulation
Nuno Borges
Protein stabilization in Hyperthermophiles
Pedro Lamosa
Friday (Oct 28)
Prokaryotic biodiversity in desert soils
Fred Rainey
Seminário (Auditório IBILI)
Microbiology of Mars analogs
Fred Rainey
Thursday (Nov 03)
Industrial (White) Biotechnology, a chance for sustainable technologies
Garo Antranikian
Seminário (Auditório IBILI)
Biocatalysis under extreme conditions;  Harvesting the potential of Extremophiles
Garo Antranikian

Affiliation and contacts
Pedro Lamosa, PhD, Investigador - Cell Physiology and NMR Group, Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, 2780-156 Oeiras, Portugal. Webpage: http://www.itqb.unl.pt/~cp-nmr/index.html
Lamosa P, Burke A, Peist R, Huber R, Liu MY, Silva G, Rodrigues-Pousada C, LeGall J, Maycock C, Santos H. (2000) Thermostabilization of proteins by diglycerol phosphate, a new compatible solute from the hyperthermophile Archaeoglobus fulgidus. Appl. Environ. Microbiol. 66:1974-9.
Lamosa P, Turner DL, Ventura R, Maycock C, Santos H. (2003) Protein stabilization by compatible solutes. Effect of diglycerol phosphate on the dynamics of Desulfovibrio gigas rubredoxin studied by NMR. Eur. J. Biochem. 270:4606-14.
Nuno Borges, PhD, Investigador - Cell Physiology and NMR Group, Instituto de Tecnologia Química e Biológica,
Universidade Nova de Lisboa, 2780-156 Oeiras, Portugal. Webpage: http://www.itqb.unl.pt/~cp-nmr/index.html
Borges N, Marugg JD, Empadinhas N, da Costa MS Santos H. (2004) Specialized roles of the two pathways for the synthesis of mannosylglycerate in osmoadaptation and thermoadaptation of Rhodothermus marinus. J. Biol. Chem. 279:9892-9898.
Borges N, Ramos A, Raven ND, Sharp RJ, Santos H. (2002) Comparative study of the thermostabilizing properties of mannosylglycerate and other compatible solutes on model enzymes. Extremophiles 6:209-16.
Fred Rainey, PhD, Professor - Department of Biological Sciences, Louisiana State University, Baton Rouge, Louisiana 70803, USA. Webpage: http://www.biology.lsu.edu/webfac/frainey/raineylab/index.php
Navarro-Gonzalez R, Rainey FA, Molina P, Bagaley DR, Hollen BJ, de la Rosa J, Small AM, Quinn RC, Grunthaner FJ, Caceres L, Gomez-Silva B, McKay CP. (2003). Mars-like soils in the Atacama Desert, Chile, and the dry limit of microbial life. Science 302: 1018-21.
Rainey FA, Ray K, Ferreira M, Gatz BZ, Nobre MF, Bagaley D, Rash BA, Park MJ, Earl AM, Shank NC, Small AM, Henk MC, Battista JR, Kampfer P, da Costa MS. (2005) Extensive diversity of ionizing-radiation-resistant bacteria recovered from Sonoran Desert soil and description of nine new species of the genus Deinococcus obtained from a single soil sample. Appl. Environ. Microbiol. 71:5225-35.
Garo Antranikian, PhD, Professor - Technical Microbiology, Technical University of Hamburg-Harburg, D-21073, Hamburg, Germany. Webpage: http://www.technical-microbiology.com/en/welc.html
Fütterer O, Angelov A, Liesegang H, Gottschalk G, Schleper C, Schepers B, Dock C, Antranikian G, Liebl W. (2004) Genome sequence of Picrophilus torridus and its implications for life around pH 0. Proc. Nat. Acad. Sci. USA 101: 9091-9096.
Deppe U, Richnow HH, Michaelis W, Antranikian G. (2005) Degradation of crude oil by an arctic microbial consortium. Extremophiles. Jul 6.
Gödde C, Sahm K, Brouns, SJ, Kluskens LD, van der Oost J, de Vos WM, Antranikian G. (2005) Cloning and expression of islandisin, a new thermostable subtilisin from Fervidobacterium islandicum, in Escherichia coli. Appl. Environ. Microbiol. 71:3951-3958.
Coordinator Milton da Costa
Santos H and da Costa MS. (2002) Compatible solutes of organisms that live in hot saline environments. Environ Microbiol. 4:501-9. Review
Silva Z, Alarico S, Nobre A, Horlacher R, Marugg J, Boos W, Mingote AI, da Costa MS. (2003) Osmotic adaptation of Thermus thermophilus RQ-1: lesson from a mutant deficient in synthesis of trehalose.J. Bacteriol. 185:5943-52.
Albuquerque L, Simoes C, Nobre MF, Pino NM, Battista JR, Silva MT, Rainey FA, da Costa MS. (2005) Truepera radiovictrix gen. nov., sp. nov., a new radiation resistant species and the proposal of Trueperaceae fam. nov. FEMS Microbiol Lett. 247:161-9.
Coordinator Nuno Empadinhas
Empadinhas N, Marugg JD, Borges N, Santos H, da Costa MS. (2001) Pathway for the synthesis of mannosylglycerate in the hyperthermophilic archaeon Pyrococcus horikoshii. Biochemical and genetic characterization of key enzymes. J. Biol. Chem. 276: 43580-8.
Empadinhas N, Albuquerque L, Costa J, Zinder SH, Santos MAS, Santos H, da Costa MS. (2004) A gene from the mesophilic bacterium Dehalococcoides ethenogenes encodes a novel mannosylglycerate synthase. J. Bacteriol. 186:4075-84.

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