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Molecular Biotechnology
 
October 3 - 7
Coordinators: Paula Veríssimo, Arthur Moir and Carlos Faro

Course Schedule
 
Monday
Tuesday
Wednesday
Thursday
Friday
9-10
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Lect. 3
Lect. 5
Lect. 7
Overview of Pratical sessions
10-11
Lect. 1
Lab.
Lab.
Lab.
 
11-12
Lab
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Lab.
 
12-13
Lab
Lab.
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14-15
Lect. 2
Lect. 4
Lect. 6
Lect. 8
 
15-16
Lab.
Lab.
Lab.
Lab.
 
16-17
Lab.
Lab.
Lab.
Lab.
 
17-18
Lab.
Lab.
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Lab.
 


Lectures
Monday, October 3
 
Lect.1: Cloning strategies
 
Lect. 2: Expression of cloned genes in prokaryotes
Tuesday, October 4
 
Lect. 3: Recombinant protein production
 
Lect. 4: Protein purification
Wednesday , October 5
 
Lect. 5: Protein characterisation I
 
Lect. 6: Protein characterisation II
Thursday, October 6
 
Lect. 7: Protein engineering
 
Lect. 8: Proteomics in health and disease
Pratical course
 
Purification and characterisation of native proteins.
 
Direct mutagenesis and expression of fusion proteins.
 
Expression, purification and characterisation of recombinant proteins.
 
 
 
Proteomics / Bioinformatics
Readings
Reading Lists
 
 
 
 
 
 
Veríssimo et al (1996) Purification, characterization and partial amino acid sequencing of two novel aspartic proteinases of fresh flowers of Cynara cardunculus L. Eur. J. Biochem. 235, 762–768.  
 
 
 
 
 
Waller LN , MJ Stump, KF Fox, WM Harley, A Fox, GC Stewart and M. Shahgholi. (2005) Identification of a second collagen-like glycoprotein produced by Bacillus anthracis and demonstration of associated spore-specific sugars. J.Bacteriol. vol. 187 Nº 3 pp 4592-4597.

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