Monday – Thursday: IBILI Auditorium
Friday : Faculty of Medicine

Coordinators: Carlos B. Duarte (CNC) & Edgar R. Gomes (Columbia University, NY)

Lecturers:
Gregg G. Gundersen
Dep. Anatomy and Cell Biology, Columbia University
New York, USA
http://cpmcnet.columbia.edu/dept/gsas/anatomy/Faculty/Gundersen/
Geri Kreitzer
Dept. of Cell and Developmental Biology 
Weill Medical College of Cornell University
New York, USA
http://www.med.cornell.edu/research/gkreitzer/index.html
Edgar R. Gomes
Dep. Pathology, Columbia University
New York, USA

Helder Maiato
IBMC, Universidade do Porto
Porto

Time – Lecture

10-11:30 – A tale of three filaments (part I) (Gregg Gundersen)

11:30 – Lunch break

13:15 - A tale of three filaments (part II) (Gregg Gundersen)
14:45 – Break
15:15 – Techniques in Cell Biology (part I) (Edgar Gomes)
16:15 – Break
16:30 - Techniques in Cell Biology (part II) (Geri Kreitzer)

Time – Lecture

8:30 – Cell Migration I (Edgar Gomes)
10:00 – Break
10:30 – Cell Migration II (Gregg Gundersen)

12:00 – Lunch Break

14:00 – Journal Club
Paper 2:
N R Adames and J A Cooper (2000) Microtubule interactions with the cell cortex causing nuclear movements in Saccharomyces cerevisiae. J Cell Biol. 149, 863-874. - [ver Pdf] (Students presenting: Ana Burgeiro, Alexandra Rosa)
Paper 3:
Hsin-Yi Henry Ho et al. (2004) Toca-1 mediates Cdc42-dependent actin nucleation by activating the N-WASP-WIP complex. Cell. 118, 203-216.- [ver Pdf] (Students presenting: Ana Raquel Soares, Vera Ribeiro)

Time – Lecture

8:30 – Journal Club:
Paper 5:
Stephanie L Gupton et al. (2005) Cell migration without a lamellipodium: translation of actin dynamics into cell movement mediated by tropomyosin. J. Cell Biol. 168, 619-631. - [ver Pdf] (Students presenting: Daniel Nunes, Joana Neves)

10:00 – Break
10:30 – Mitosis I (Helder Maiato)

12:00 – Lunch Break

14:00 – Mitosis II (Helder Maiato)
15:30 – Journal Club
Paper 7:
Tarun M Kapoor et al. (2006) Chromosomes can congress to the metaphase plate before biorientation. Science. 311, 388-391. - [ver Pdf]
A Khodjakov et al (1997) Chromosome fragments possessing only one kinetochore can congress to the spindle equator. J. Cell Biology. 136, 229-240. - [ver Pdf]
(Students presenting: Ana Raquel Esteves, Gil Costa, Sara Sousa)

Paper 8:
Olivier Pertz et al. (2006) Spatiotemporal dynamics of RhoA activity in migrating cells. Nature. 440, 1069-1072. - [ver Pdf] (Students presenting: Joana Gaspar, Rui Costa)

Time – Lecture

9:00 – Protein sorting and vesicular trafficking (Geri Kreitzer)
10:30 – Break
10:45 – Journal Club
Paper 11
R D Vale, T S Reese, and M P Sheetz (1985) Identification of a novel force-generating protein, kinesin, involved in microtubule-based motility. Cell. 42, 39-50. - [ver Pdf]
S T Brady, R J Lasek, and R D Allen (1982) Fast axonal transport in extruded axoplasm from squid giant axon. Science. 218, 1129-1131. - [ver Pdf]
(Students presenting: Daniela Arduíno, Marina Brito)

12:00 Lunch Break

14:45 – Seminar
Centrosome polarization in migrating cells (Edgar Gomes)
15:45 – Break
16:00 – Seminar
Functional Analysis of Kinesins during Epithelial Polarization
(Geri Kreitzer)

11:00 – Journal Club
Paper 9
Shigenori Nonaka et al. (1998) Randomization of left–right asymmetry due to loss of nodal cilia generating leftward flow of extraembryonic fluid in mice lacking KIF3B motor protein. Cell 95, 829-837. - [ver Pdf] (Students presenting: Joana Ferreira, Pedro Branco)

12:30 – Lunch Break

14:00 – Journal Club
Paper 10
Froylan Calderon de Anda et al. (2005) Centrosome localization determines neuronal polarity. Nature. 436, 704-708. - [ver Pdf]
C G Dotti and G Banker (1991) Intracellular organization of hippocampal neurons during the development of neuronal polarity. J. Cell Sci. Suppl. 15, 75-84. - [ver Pdf]
(Students presenting: Marta Vieira, Sofia Grade, Tatiana Catarino)

 

Enrique Rodriguez-Boulan, Geri Kreitzer and Anne Müsch (2005) Organization of vesicular trafficking in epithelia. Nature Rev Mol Cell Biol 6, 233-247.

Gregg G. Gundersen (2002) Evolutionary conservation of microtubule-capture mechanisms. Nature Rev Mol Cell Biol 3, 6-14.

Myrto Raftopoulou and Alan Hall (2004) Cell migration: Rho GTPases lead the way. Dev. Biol 265, 23-32.

Thomas Pollard and Gary Borisy (2003) Cellular motility driven by assembly and disassembly of actin filaments. Cell. 112, 453-465.