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NMR
 
February 26 - March 2
Carlos Geraldes, Margarida Castro, Rui Carvalho, John Jones
Area: Novas técnicas de aplicação em Biomedicina
Duração: 26 February 2007 - 2 March 2007

Coordinators: Carlos Geraldes, Margarida Castro, Rui Carvalho, John Jones
                      (CNC, Coimbra)

Lecturers:
Carlos F. Geraldes, Ph.D. - Department of Biochemistry, FCT, University of Coimbra
Margarida Castro, Ph.D. - Department of Biochemistry, FCT, University of Coimbra
Rui Carvalho, PhD . - Department of Biochemistry, FCT, University of Coimbra
John Jones, D.Sc. - CNC, Coimbra
Bernardo Celda Muñoz, Ph.D. - Department of Chemistry, Universitat de Valencia, Spain      
Shawn Burgess, D.Chem. - UT Southwestern Medical Center at Dallas, Dallas, Texas, USA
Silvio Aime, Ph.D. - Chemistry Department and Center for Molecular Imaging, University of Torino, Torino, Italy

Course Schedule
Monday : Introduction and Background
10.00 -11.30 h           
Introduction to Biomedical NMR Spectroscopy: basic parameters and their information content
Carlos F. Geraldes

11.30-13.00 h           
Introduction to Magnetic Resonance Imaging (MRI)
                                   
Carlos F. Geraldes

15.00-17.00 h           
Tutorials
Carlos Geraldes, Margarida Castro, Rui Carvalho, John Jones

Tuesday: NMR methodologies for metabolic studies
9.00 -10.00 h           
Introduction to multinuclear NMR studies in cells and body fluids
Margarida Castro

10.30-11.30 h           
Introduction to the study of intermediary metabolism by NMR
Rui Carvalho

14.30-15.30 h          
Clinical Applications of 2H and 13C NMR
John Jones

16.00-17.00 h           
Tutorials
Rui Carvalho, Margarida Castro, John Jones

Wednesday: Mouse disease models
9.00 -10.00 h        
Metabolic phenotyping of mice by NMR
Shawn Burgess

10.30-11.30 h
           
NMR characterization of liver metabolism in mutant mice
Shawn Burgess

14.30-17.00 h
        
Analysis and discussion of papers by the students:

Impaired Tricarboxylic Acid Cycle Activity in Mouse Livers Lacking Cytosolic Phosphoenolpyruvate Carboxykinase, Shawn C. Burgess, Natasha Hausler, Matthew Merritt, F. Mark H. Jeffrey, Charles Storey, Angela Milde, Seena Koshy, Jill Lindner, Mark A. Magnuson, Craig R. Malloy and A. Dean Sherry, J. Biol. Chem., 279, 48941– 48949, 2004
Marina Brito e Daniel Farinha – discussion by Shawn Burgess
9.00-10.00 h:             
Brain and liver tissue metabolite profiles by in vivoMetabolite identification in human liver needle biopsies by high-resolution magic angle spinning 1H NMR spectroscopy, Beatriz Martınez-Granados, Daniel Monleon, M. Carmen Martınez-Bisbal, Jose Manuel Rodrigo, Juan del Olmo, Paloma Lluch, Antonio Ferrandez, Luis Martı-Bonmatı and Bernardo Celda, NMR Biomed. 18, 1–11, 2005.
Pedro Branco e Ana Soares - discussion by Bernardo Celda
and ex vivo NMR
Bernardo Celda

10.30-11.30 h:           
Brain tumour and liver diffuse pathologies classification by HR-MAS and MRS
Bernardo Celda

14.30-17.30 h:           
Analysis and discussion of papers by the students:

Highly Sensitive MRI Chemical Exchange Saturation Transfer Agents Using Liposomes, Silvio Aime, Daniela Delli Castelli and Enzo Terreno, Angew. Chem. Int. Ed. 44, 5513 –5515, 2005.
Vera Ribeiro e Sara Sousa – discussion by Silvio Aime
Effect of the Intracellular Localization of a Gd-Based Imaging Probe on the Relaxation Enhancement of Water Protons, Enzo Terreno, Simonetta Geninatti Crich, Simona Belfiore, Luigi Biancone, Claudia Cabella, Giovanna Esposito, Andrea D. Manazza, and Silvio Aime, Magnetic Resonance in Medicine, 55, 491–497, 2006.
Joana Gaspar e Joana Neves – discussion by Silvio Aime



Friday: Molecular Imaging by NMR
9.00-10.00 h           
Multimodal Molecular Imaging Agents
Silvio Aime

10.30-11.30h       
Responsive and Targeting MRI Contrast Agents
Silvio Aime
Friday Seminar - 16.00 h                       
Magnetic Resonance Contrast Agents for Molecular Imaging Applications
Silvio Aime


Invited lecturers
1. Bernardo Celda, Ph.D.
Department of Physical Chemistry, Laboratory of Molecular Imaging (UCIM/SCSIE)
Universitat de Valencia
46100-Burjassot (Valencia)- Spain
e.mail: bernardo.celda@uv.es
Papers:
[1] M.C. Martínez-Bisbal, L. Martí-Bonmatí, J. Piquer, A. Revert, P. Ferrer, J.L. Llácer, M. Piotto, O. Assemat, B. Celda. 1H and 13C HR-MAS spectroscopy of intact biopsy samples ex-vivo and in-vivo 1H MRS study of human high grade gliomas. NMR in Biomedicine 17: 191-205 (2004)
[2] B. Martínez-Granados, D. Monleon, MC Martínez-Bisbal, JM. Rodrigo, J. del Olmo, P. Lluch, A. Ma Ferrandez, B. Celda Metabolite identification in human liver needle biopsies by high-resolution magic angle spinning 1H NMR spectroscopy. NMR in Biomedicine 18: 1-11 (2006)

2. Shawn Burgess, D.Chem.
The Mary Nell and Ralph B. Rogers Magnetic Resonance Center
University of Texas, Southwestern Medical Center at Dallas
75235-9085 Dallas, Texas, USA
Email: Shawn.Burgess@UTSouthwestern.edu

Papers:
[1] Shawn C. Burgess, Natasha Hausler, Matthew Merritt, F. Mark H. Jeffrey, Charles Storey,Angela Milde, Seena Koshy, Jill Lindner, Mark A. Magnuson, Craig R. Malloy,and A. Dean Sherry. Impaired tricarboxylic acid cycle activity in mouse livers lacking cytosolic phosphoenolpyruvate carboxykinase. J. Biol. Chem. 279: 48941-48949 (2004).
[2] Shawn C. Burgess, Teresa C. Leone, Adam R. Wende, Michelle A. Croce, Zhouji Chen, A. Dean Sherry, Craig R. Malloy, and Brian N. Finc. Diminished Hepatic Gluconeogenesis via Defects in TCA Cycle Flux in PPARγ Coactivator-1α (PGC-1α)-Deficient Mice. J. Biol. Chem. 281: 19000-19008 (2006).

3. Silvio Aime, Ph.D.
Chemistry Department and Center for Molecular Imaging,
University of Torino, Torino, Italy
Email: silvio.aime@unito.it
Papers:
[1]  Effect of the intracellular localization of a Gd-based imaging probe on the relaxation enhancement of water protons, Terreno E, Crich SG, Belfiore S, Biancone L, Cabella C, Esposito G, Manazza AD, Aime S, Magnetic Resonance in Medicine, 55: 491-497 (2006).
[2]  Highly sensitive MRI chemical exchange saturation transfer agents using liposomes, Aime S, Castelli DD, Terreno E, Angewandte Chemie-Int. Edition, 44: 5513-5515 (2005)
 
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