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Structural Biology
 
October 9 - 13
Ulrich Baumann, Christina Redfield, Rui Brito and Sandra Ribeiro

Invited Lecturers:
 
 
Ulrich Baumann (PhD), Department of Chemistry & Biochemistry, University of Berne, Switzerland
 
 
 
 
 
Christina Redfield (PhD) Oxford Centre for Molecular Sciences, University of Oxford, England
 
 
 
 
Specific title: Protein Structure: Why study it? How to study it?

Objectives:
A complete understanding of cellular and systems biology requires the detailed structural understanding of hundreds of biological machines. This goal demands a concerted effort of complementary biochemical and biophysical approaches for the systematic identification, isolation, structural and dynamic characterization, and mechanistic analysis of multi-component systems.
 
The objective of the present course is to highlight the role of structural biology and biophysical chemistry in the elucidation of the three-dimensional structure and dynamics of proteins. A particular focus will be given to the determination of the three-dimensional structures of proteins by X-ray crystallography and NMR.
Course Schedule
Monday (Oct 09)
9:30-10:30
Proteins, molecular machines. Interplay between structure and function.
Tools and techniques to study protein structure I
 
Rui Brito
10:45-11:45
Why study protein structure? Tools and techniques to study protein structure II
 
Sandra Ribeiro
 
 
14:30-15:30
X-ray crystallography I
 
Ulrich Baumann
 
 
15:45-17:45 
 
Sandra Ribeiro & Rui Brito
 
Groups I, II and III: Paper preparation
 
Ulrich Baumann
 
 
Tuesday (Oct 10)
9:30-10:30
X-ray crystallography II
 
Ulrich Baumann
10:45-11:45
X-ray crystallography III
 
Ulrich Bauman
 
 
14:30-16:30
 
Sandra Ribeiro Rui Brito
 
Groups IV, V, and VI: Paper preparation
 
Christina Redfield
 
 
 
 
Wednesday (Oct 11)
9:30-10:30 
Introduction to NMR of Proteins: Basic Principles
 
Christina Redfield
10:45-11:45
font size="2">Introduction to NMR of Proteins: Spectral Assignment
 
Christina Redfield
 
 
14:00-16:00
Journal Club I (30 min each group)
 
 
 
16:15-17:00
SEMINAR
On the membrane-side of protein destruction
 
Ulrich Baumann
 
 
Thursday (Oct 12)
9:30-10:30 
Determination of Protein Structure by NMR
 
Christina Redfield
10:45-11:45
Using NMR to Study Ligand Binding and Protein-Protein Interactions
 
Christina Redfield
 
 
14:30-17:30
Journal Club II (30 min each group)
 
 
 
GROUP IV:  Solution Structure of the Hdm2 C2H2C4 RING, a Domain Critical for Ubiquitination of p53,
 
 
 
GROUP VI: Structural Consequences of Cysteine Substitutions C1977Y and C1977R in Calcium-binding Epidermal Growth Factor-like Domain 30 of Human Fibrillin-1
 
 
 
Friday (Oct 13)
9:30-11:00
Using NMR to Probe Protein Folding and Dynamics
 
Christina Redfield
 
 
16:00-17:00
SEMINAR Using NMR to study calcium binding in modular proteins containing epidermal growth factor-like (EGF) domains  
 
Christina Redfield
 
 
 
 
Complementary reading
7- TROSY and CRINEPT: NMR with large molecular and supramolecular structures in solution
8- NMR and Structural Genomics
9- Residual Dipolar Couplings in Structure Determination of Biomolecules
10- Using NMR Spectroscopy to study molten globule states of proteins
11- New methods of structure refinement for macromolecular structure determination by NMR


Publications of invited speakers and coordinators
Invited speakers
 
 
Ulrich Baumann
 
 
 
 
 
 
Christina Redfield
 
 
 
 
 
 
Coordinators
 
Sandra Macedo-Ribeiro
 
 
 
 
 
 
 
Rui M. M. Brito
 
 
A structural model of an amyloid protofilament of Transthyretin.
 
How important is the role of compact denatured states on amyloid formation by transthyretin?
 
Tetramer Dissociation and Monomer Partial Unfolding Precedes Protofibril Formation in Amyloidogenic Transthyretin Variants.
 
Amyloid Formation by Transthyretin: From Protein Stability to Protein Aggregation

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