Home
About BEB
Curriculum
Admission
CNC Research
BEB in the Media
 
 
Retinal Physiology and Disease: from Bench to Clinics
 
February 4 - 8
Miguel Castelo-Branco, Francisco Ambrósio
Programa Doutoral em Biologia Experimental e Biomedicina
(PDBEB)
Doctoral Programme in Experimental Biology and Biomedicine
Center for Neuroscience and Cell Biology (CNC)
[Faculty of medicine, Polo I, 1st floor]
 
1. Course TitleRetinal Physiology and Disease: from Bench to Clinics

2. Scientific AreaNeuroscience

3. Duration and Dates5 days
4 - 8 February 2008

4. Organizors
António Francisco Ambrósio
Center of Ophthalmology of Coimbra, IBILI
Faculty of Medicine, University of Coimbra
3000-548 Coimbra
Portugal
Phone: +351239480222
Fax: +351239480280
Email: fambrosio@ibili.uc.pt
[Curriculum Vitae]
Miguel Castelo-Branco
Visual Neuroscience Laboratory
Center of Ophthalmology of Coimbra, IBILI
Faculty of Medicine, University of Coimbra
3000-548 Coimbra
Portugal
Phone +351 239480286
Fax +351 239480280
mcbranco@ibili.uc.pt
mcbranco@ci.uc.pt

5. Invited Lecturers
Serge Picaud
INSERM U-592, Université Pierre et Marie Curie-Paris6, Laboratoire de Physiopathologie Cellulaire et Moleculaire de la Retine, F-75571 Paris
France.
Email: serge.picaud@st-antoine.inserm.fr
Ulrich Schraermeyer
University Hospital of Tübingen - Clinic of Ophthalmology - Section of Experimental Vitreoretinal Surgery
Schleichstraße 12/1
72076 Tübingen
Germany
Phone: +49-7071-2980715
Fax: +49-7071-294554
E-mail: ulrich.schraermeyer@med.uni-tuebingen.de

Espen Hartveit
Department of Biomedicine, University of Bergen, Jonas Lies vei 91, 5009 Bergen
Norway
Phone: +47-55586350 (office) / +47-55586047 (lab.)
Fax: +47-55586360.
Email: espen.hartveit@biomed.uib.no

6. Program
Main goals of the course
To understand retinal function, as a model to study sensory systems.
To study the mechanisms underlying retinal degenerative diseases and evaluate the potential of new therapeutic strategies.

Day 1
Monday, February 4
Morning
9:15 – 9:30 Introductory notes
Francisco Ambrósio and Miguel Castelo-Branco
9:30 – 11:00 - Lecture 1
The retina: understanding the puzzle
Francisco Ambrósio
11:30 – 13:00 - Lecture 2
Basic principles of organization of the visual system: from the retina to the cortex
Miguel Castelo-Branco
Afternoon
14:30 – 15:30
Challenges in choosing a lab and a PhD project within the scope of a Doctoral Program
Discussion with Faculty members
Paper and group assignments for discussion

Day 2
Tuesday, February 5

Morning
9:15 - 10:15 - Lecture 1
Photoreceptor degenetarion: From single cell and multielectrode recordings of retinal cells to therapeutic strategies
Serge Picaud
10:15 – 11:15 - Lecture 2
Diabetic retinopathy: beyond microvascular dysfunction
Francisco Ambrósio
11:45 – 12:45 - Lecture 3
Does GABA excitatory transmission in the adult retina contribute to retinal pathophysiology?
Serge Picaud

Afternoon
14:30 – 16:30
Chairpersons – Serge Picaud and Francisco Ambrósio
Paper presentations and discussion (2 papers - References from Francisco Ambrósio and Serge Picaud)

Day 3
Wednesday, February 6
Morning
9:15 – 10:15
Chairperson – Serge Picaud
Paper presentation and discussion (1 paper - Reference from Serge Picaud)
10:15 – 11:15 - Lecture 1
New therapeutic approaches to treat retinal degenerations: pros and cons.
Ulrich Schraermeyer
11:45 – 12:45 - Lecture 2
Structure-Function relationships in the visual system. Implications for genotype-phenotype relationships in retinal and cortical disorders
Miguel Castelo-Branco
Afternoon
14:30 – 15:30 - Lecture 3
Stem cells: a tool to rescue the retina
Ulrich Schraermeyer
15:30 – 16:30
Chairperson – Miguel Castelo-Branco
Paper presentation and discussion (1 paper - Reference from Miguel Castelo-Branco)

Day 4
Thursday, February 7

Morning
9:15 – 10:30 - Lecture 1
Electrical synapses in the rod pathway of the mammalian retina
Espen Hartveit
11:00 – 12:15 - Lecture 2
Glycinergic input to rod bipolar cells and AII amacrine cells of the mammalian retina
Espen Hartveit
Afternoon
14:00 – 16:00
Chairperson – Ulrich Schraermeyer
Paper presentations and discussion (2 papers - References from Ulrich Schraermeyer)

Day 5
Friday, February 8
Morning
9:15 – 12:00
Chairperson – Espen Hartveit
Paper presentations and discussion (2 papers - References from Espen Hartveit).
Afternoon
16:00 - Seminar:
Regulation of synaptic transmission through presynaptic glutamate transporters in the retina
Espen Hartveit

7. Relevant Publications of the Lecturers
Serge Picaud
M. Frasson, J.A. Sahel, M. Fabre, M. Simonutti, H. Dreyfus and S. Picaud. Retinitis pigmentosa: rod photoreceptor rescue by a calcium-channel blocker in the rd mouse. Nat Med 1999, 5:1183-1187.
S. Picaud, H.P. Larsson, D.P. Wellis, H. Lecar and F. Werblin. Cone photoreceptors respond to their own glutamate release in the tiger salamander. Proc Natl Acad Sci U S A 1995, 92:9417-9421.
Ulrich Schraermeyer
S. Arnhold, P. Heiduschka, H. Klein, Y. Absenger, S. Basnaoglu, F. Kreppel, S. Henke-Fahle, S. Kochanek, K.U. Bartz-Schmidt, K. Addicks and U. Schraermeyer. Adenovirally transduced bone marrow stromal cells differentiate into pigment epithelial cells and induce rescue effects in RCS rats. Invest Ophthalmol Vis Sci 2006, 47:4121-4129.
I. Semkova, F. Kreppel, G. Welsandt, T. Luther, J. Kozlowski, H. Janicki, S. Kochanek and U. Schraermeyer. Autologous transplantation of genetically modified iris pigment epithelial cells: a promising concept for the treatment of age-related macular degeneration and other disorders of the eye. Proc Natl Acad Sci U S A 2002, 99:13090-13095.
Espen Hartveit
E Hartveit, ML Veruki. Studying properties of neurotransmitter receptors by non-stationary noise analysis of spontaneous postsynaptic currents and agonist-evoked responses in outside-out patches. Nat Protoc. 2007, 2:434-448.
ML Veruki, SH Morkve, E Hartveit. Activation of a presynaptic glutamate transporter regulates synaptic transmission through electrical signaling. Nat Neurosci. 2006, 9:1388-96.


TUTORIAL
and
Paper and Group Assignments
Course Title:
Retinal Physiology and Disease: from Bench to Clinics
Organizers: António Francisco Ambrósio e Miguel Castelo-Branco
The Course aims giving an overview of the anatomy and physiology of the retina. Particularly, the importance of main cell types and their involvement in the function of retinal circuits will be addressed. Also, the Course will focus on the pathophysiological mechanisms underlying retinal dysfunction and degeneration, and therapeutic strategies designed to treat retinal disorders will also be discussed.
The students must attend all lectures. During the course, 8 selected papers will be presented by the students and discussed (1 or 2 students/paper). The students are asked to make the presentations using Power Point, and the duration will be 15 min maximum! In general, the Power Point presentation should have 1/2 slides for Introduction and Objectives, 1 for Conclusions and Future Directions, and the others for the paper figures. Students are supposed to offer a critical view of the paper and not a mere description. Also, students are welcome to ask all lecturers for advice, including matters not directly related to the Course, such as possible contacts for a PhD, letters of recommendation, etc. The students must read carefully all papers that will be presented, and are invited to read additional selected papers and a few reviews that will certainly help to better understand the original papers that will be presented.

The papers will be presented in the following order:

Tuesday, Feb. 5, afternoon
Chairperson – Francisco Ambrósio
Paper 1 – Diabetes reduces basal retinal insulin receptor signalling. Reiter et al., Diabetes 2006, 55, 1148.

Students: Nélio Gonçalves

Tuesday, Feb. 5, afternoon
Chairperson – Serge Picaud
Paper 2 – Identification and characterization of rod-derived cone viability factor. Leveillard et al., Nat Genet. 2004, 36:755.
Students: Susana Louros and Filipe Leal

Wednesday, Feb. 6, morning
Chairperson - Serge Picaud
Paper 3 – Apoptosis in retinal degeneration involves cross-talk between apoptosis-inducing factor (AIF) and caspase-12 and is blocked by calpain inhibitors. Sanges et al., Proc Natl Acad Sci U S A. 2006, 103:17366.
Students: Joana Marinho and João Noutel

Thursday, Feb. 7, afternoon
Chairperson – Ulrich Schraermeyer
Paper 5 – Adenovirally Transduced Bone Marrow Stromal Cells Differentiate into Pigment Epithelial Cells and Induce Rescue Effects in RCS Rats. Arnhold et al., Invest Ophthalmol Vis Sci. 2006;47:4121. 2006;47:4121
Students: Sara Figueiredo and Sueli Marques

Thursday, Feb. 7, afternoon
Chairperson – Ulrich Schraermeyer
Paper 6 – Neurally Selected Embryonic Stem Cells Induce Tumor Formation after Long-Term Survival following Engraftment into the Subretinal Space. Arnhold et al., Invest Ophthalmol Vis Sci. 2004;45:4251.
Students: Sofia Azevedo e Sofia Cabral

Friday, Feb. 8, morning
Chairperson – Espen Hartveit
Paper 7 – Fast neurotransmitter release triggered by Ca influx through AMPA-type glutamate receptors. Chavez et al., Nature 2006, 443:705.
Students: Lígia Silva

Friday, Feb. 8, morning
Chairperson – Espen Hartveit
Paper 8 – Activation of a presynaptic glutamate transporter regulates synaptic transmission through electrical signaling. Veruki et al., Nat Neurosci. 2006, 9:1388.
Students: Filipa Baptista



The organizers also suggest the lecturers and the students to have lunch together everyday and to meet on Tuesday evening for dinner.



Supplementary Reading
back
 
© 2017 BEB - Doctoral Programme in Experimental Biology and Biomedicine powered by ponto.C