Synapse structure and function – PDBEB course
Date: March 22-26, 2021
Location: CNC-Center for Neuroscience and Cell Biology, University of Coimbra
Ana Luísa Carvalho (email@example.com); CNC & FCTUC, University of Coimbra
Carlos B. Duarte (firstname.lastname@example.org); CNC & FCTUC, University of Coimbra
Paulo Pinheiro (email@example.com); CNC, University of coimbra
Ana Luísa Cardoso, CNC, University of Coimbra ORCID
Ana Luísa Carvalho, CNC & FCTUC, University of Coimbra ORCID; Lab
Ângela Inácio, CNC, University of Coimbra
Carlos B. Duarte, CNC & FCTUC, University of Coimbra ORCID; Lab
Dominique Fernandes, CNC, University of Coimbra
Ira Milosevic, University of Oxford, Wellcome Centre for Human Genetics,
Nuffield Department of Medicine & CNC, University of Coimbra ORCID, Lab
João Peça, CNC & FCTUC, University of Coimbra ORCID; Lab
Miranda Mele, CNC, University of Coimbra
Paulo Pinheiro, CNC, University of Coimbra ORCID; Lab
Ramiro D. Almeida, iBIMED, University of Aveiro & CNC, University of Coimbra ORCID
Ricardo Rodrigues, CNC, University of Coimbra ORCID; Lab
Rui O. Costa, CNC, University of Coimbra
Christophe Mulle (IINS, University of Bordeaux)
Daniel Finely (Harvard Medical School)
9.00h-9.15h: Structure of the course; Introduction of students and lecturers
9.15h-10.30h: Neuronal migration: inter- and intracellular signaling. Ricardo Rodrigues
10.45h-11.45h: Establishment of neuronal polarity and axon pathfinding – Rui O. Costa
12.00h-13.30h: Electrophysiology: a primer (I) - João Peça
14.30h-15.45h: Electrophysiology: a primer (II) - Paulo Pinheiro
Neuronal polarity and axon pathfinding
9.00h-10.00h: Molecular triggers of synapse formation and maturation – Ramiro D. Almeida
10.15h-11.15h: Introduction to synapses: structure-function and molecular composition - Ana Luisa Carvalho
11.30 -12.30h: Introduction to the presynapse - Paulo Pinheiro
14.00h-15.00h: Methods for studying presynaptic function – Paulo Pinheiro
9.00h-10.00h: Modes for synaptic vesicle formation, acidification and neurotransmitter filling – Ira Milosevic
10.15h-11.15h: Synaptic vesicle maturation and exocytosis – Ira Milosevic
11.30 -12.15h: Postsynaptic excitatory receptor function - Ana Luisa Carvalho
12.30h-13.15h: Synaptic plasticity & molecular mechanisms -Ana Luísa Carvalho
14h: Seminar by Christophe Mulle (IINS, University of Bordeaux)
Presynaptic plasticity in hippocampal circuits
Exocytosis and Endocytosis
9.00h-10.00: Inhibitory synapse function and plasticity – Miranda Mele
10.15h-11.15h: Synaptic sculpting by microglia in health and disease – Ana Luisa Cardoso
11.30h-12.30h: Synaptic modulation by neurotrophins - Carlos B. Duarte
Lab talks (Zoom link):
13.30h-13.50h: Disrupted synaptic transmission and plasticity upon genetic or antibody-mediated disruption of Caspr2 – Dominique Fernandes
13.50h-14.20h: Synaptic alterations in an animal model of intellectual disability – Ângela Inácio
9.00h-11.00h: Grant presentation by students and discussion (see below)
14h: Seminar by Daniel Finley (Department of Cell Biology, Harvard Medical School) Global remodeling of the proteome during extreme terminal differentiation
Students (in groups) will prepare a grant proposal based on original papers proposed to them.
On March 26th, students will present and discuss their proposal.
Throughout the course the students should approach their tutor to discuss the grant proposal.
Groups 1 and 2 (Zoom link) – tutor Ana Luisa Carvalho
Group 1: Daniela Lopes, Raquel Santos, Maria Inês Alfaiate
Ribeiro LF, Catarino T, Carvalho M, Cortes L, Santos SD, Opazo PO, Ribeiro LR, Oliveiros B, Choquet D, Esteban JA, Peça J, Carvalho AL. Ligand-independent activity of the ghrelin receptor modulates AMPA receptor trafficking and supports memory formation. Sci Signal. 2021 Feb 16;14(670):eabb1953. doi: 10.1126/scisignal.abb1953. PMID: 33593997.
Group 2: Filipa Gouveia, Marta Sousa
Araki Y, Hong I, Gamache TR, Ju S, Collado-Torres L, Shin JH, Huganir RL.
SynGAP isoforms differentially regulate synaptic plasticity and dendritic development.
Elife. 2020 Jun 24;9:e56273. doi: 10.7554/eLife.56273.
Groups 3 and 4 (Zoom link) – tutor Paulo Pinheiro
Group 3: Annika-Julia Jahn, Daniel Henriques
Gowrisankaran, S., Houy, S., del Castillo, J.G.P. et al.
Endophilin-A coordinates priming and fusion of neurosecretory vesicles via intersectin.
Nat Commun 11, 1266 (2020). https://doi.org/10.1038/s41467-020-14993-8
Group 4: Carla Cavaleiro, Inês Seixas Ramos
Fujii T, Sakurai A, Littleton JT, Yoshihara M. Synaptotagmin
7 switches short-term synaptic plasticity from depression to facilitation by suppressing synaptic transmission.
Sci Rep. 2021 Feb 18;11(1):4059. doi: 10.1038/s41598-021-83397-5.
Goups 5 and 6 (Zoom link) – tutor Carlos B Duarte
Group 5: Matilde Pereira, Marianna Mekhaeil
Afonso P, De Luca P, Carvalho RS, Cortes L, Pinheiro P, Oliveiros B, Almeida RD, Mele M, Duarte CB. (2019) BDNF increases synaptic NMDA receptor abundance by enhancing the local translation of Pyk2 in cultured hippocampal neurons. Sci Signal 12(586):eaav3577.
Group 6: Miguel Rosado, Diana Lobo,
Heumüller M, Glock C, Rangaraju V, Biever A, Schuman EM. (2019) Spatially stable mitochondrial compartments fuel local translation during plasticity. Cell .176(1-2):73-84.e15.