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[CANCELLED] PDBEB Courses 2019/2020 - Synapse structure and function
 
March 23 - 27
Coordinators: Ana Luisa Carvalho - alc@cnc.uc.pt & Carlos Duarte - cbduarte@ci.uc.pt

Doctoral Program in Experimental Biology and Biomedicine (PDBEB) 2019/2020 Edition

Synapse Structure and Function @ CNC

March 23 – March 27, 2020



Registration is mandatory here.



Deadline for registration:

 March 16, 2020

Coordinators: Ana Luísa Carvalho - alc@cnc.uc.pt & Carlos B. Duarte - cbduarte@ci.uc.pt) & Paulo Pinheiro - pcspinheiro@gmail.com

Lecturers:

Ana Luísa Cardoso, CNC, University of Coimbra ORCID

Ana Luísa Carvalho, CNC & FCTUC, University of Coimbra ORCID; Lab

Carlos B. Duarte, CNC & FCTUC, University of Coimbra ORCID; Lab

Christophe Mulle, IINS-Institute for Interdisciplinary Neuroscience, University of Bordeaux, France Lab

Filipe V. Duarte, CNC, University of Coimbra

Ira Milosevic, European Neuroscience Institute (ENI), Göttingen, Germany ORCID, Lab

João Peça, CNC, University of Coimbra ORCID; Lab

Luísa Cortes, CNC, University of Coimbra ORCID

Miranda Mele, CNC, University of Coimbra

Paulo Pinheiro, CNC, University of Coimbra ORCID; Lab

Ramiro D. Almeida, iBIMED, University of Aveiro & CNC, University of Coimbra ORCID

Ricardo Rodrigues, CNC, University of Coimbra ORCID; Lab

Rui O. Costa, CNC, University of Coimbra



Date: March 23 – March 27, 2020



Location: CNC-Center for Neuroscience and Cell Biology, University of Coimbra




Program




March 23

9h00 - 9h15: Structure of the course; Introduction of students and lecturers

9h15 - 10h30: Neuronal migration: inter and intracellular signaling. Ricardo Rodrigues

10h45 - 11h45: Establishment of neuronal polarity and axon pathfinding – Rui O. Costa

14h30 - 16h00: Electrophysiology: a primer (I) - João Peça

16h15 - 17h30: Electrophysiology: a primer (II) - Paulo Pinheiro

 

March 24

9h00 - 10h00: Molecular triggers of synapse formation and maturation – Ramiro D. Almeida

10h15- 11h15: Introduction to synapses: structure-function and molecular composition - Ana Luisa Carvalho

11h30 - 12h30: Introduction to the presynapse - Paulo Pinheiro

14h00 - 15h00: Methods for studying presynaptic function – Paulo Pinheiro




March 25

9h00h-10.00h: Modes for synaptic vesicle formation, acidification and neurotransmitter filling – Ira Milosevic

10h15 - 11h15: Synaptic vesicle maturation and exocytosis – Ira Milosevic

11h30 - 12h30: Postsynaptic excitatory receptor function - Ana Luisa Carvalho

14h00 - 15h00: Synaptic plasticity & molecular mechanisms -Ana Luísa Carvalho

15h15 - 16h15: Inhibitory synapse function and plasticity – Miranda Mele




March 26

9h00 - 10h00 - Multi-photon microscopy: principles and applications; Live imaging of neurons and synapses in vivo - Luisa Cortes

10h30 - 17h30: Practical session

Two-photon microscopy - Imaging spine dynamics in organotypic hippocampal slices - Luísa Cortes

Or

Calcium imaging in cultured neuronsMiranda Mele & Filipe V. Duarte

 

 

March 27

9h00 - 10.00: Synaptic sculpting by microglia in health and disease – Ana Luisa Cardoso

10h15 - 11.15: Synaptic modulation by neurotrophins - Carlos B. Duarte

14h00: Seminar by Christophe Mulle

Presynaptic plasticity in hippocampal circuits.

 

 

Student Assignments:

Students (in groups) will write a grant proposal based on original papers that will be proposed to them. The grant proposal is 3 pages long and should include the following sections: a) State of the art; b) Objectives of proposal; 3) Proposed tasks and methodology. Throughout the course the students will meet with tutors for discussing the grant proposal. Grant Proposal Deadline – April 3, 2020

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