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PDBEB Courses 2018/2019 - Core Technologies @ CNC
January 21 - February 1
Coordinator: Luisa Cortes (lcortes@cnc.uc.pt)
Doctoral Program in Experimental Biology and Biomedicine (PDBEB) 

2018/2019 Edition

Core Technologies @ CNC

January 21 – February 1, 2019


Luísa Cortes (lcortes@cnc.uc.pt); CNC, University of Coimbra


Bruno Manadas (bmanadas@cnc.uc.pt); CNC, University of Coimbra
Conceição Egas (cegas@biocant.pt); CNC, University of Coimbra
Isabel Nunes (inunes@cnc.uc.pt); CNC, University of Coimbra
Isaura Simões (isimoes@biocant.pt); CNC, University of Coimbra
Marta Pinto (mlaranjeiro.pinto@gmail.com); CNC, University of Coimbra
Miguel Mano (mano@ci.uc.pt); CNC, University of Coimbra
Ricardo Vieira Pires (ricardo.pires@biocant.pt);CNC, University of Coimbra
Susana Pedreiro (susanapedreiro@gmail.com); CNC, University of Coimbra
Tatiana Catarino (tatianacatarino@gmail.com); CNC, University of Coimbra

Date: January 21 – February 1, 2019

UC-Biotech and CNC (FMUC, POLO I building)

The aim of this course is to provide an overview of the technological platforms available at CNC.
This two-week course will focus on the principles and high-end applications of the different methodologies and students will gain a theoretical understanding and hands-on experience on state-of-the-art equipment and on data processing and analysis.


Week I – 21st -25th January 2019 – UC-Biotech

Monday, 21th January 2019
9h30 - Welcome Session – Luisa Cortes (UC-Biotech Auditorium)
Transcriptomics to characterize cellular mechanisms: RNA-Seq (UC-Biotech)
10h00 -11h20: RNA-Seq: NGS and experimental design – Conceição Egas
11h40 – 13h00: RNA-Seq analysis. Data processing and analysis - Hugo Froufe
14h30 - 17h00: Hands on RNA extraction and QC- I

Tuesday, 22nd January 2019
Transcriptomics to characterize cellular mechanisms: RNA-Seq (UC-Biotech)
10h00 - 12h30: Hands on RNA extraction and QC- I
14h00 - 17h00: Hands on data processing and analysis

Wednesday, 23rd January 2019
High-throughput/high-content screening
9h00-10h00 – High-throughput/high-content screening: from basic principles to emerging technologies
10h00-11h00 – Case studies: RNAi screenings for gene and microRNA function
11h30-12h30 – Principles of automated image analysis
14h00-16h00 – Hands-on (sample processing, image acquisition)
16h00-18h00 – Hands-on (image analysis)

Thursday, 24th January 2019
Recombinant protein expression and purification (UC-Biotech)
09h30-10h45 – Cloning strategies: the old and the new
11h15-12h30 – Protein expression and purification: there is no such thing as a “one size fits all solution”
14h00-18h30 – Hands-on protein purification (please bring lab coats)

Friday, 25th January 2019
R&D Antibodies
9h30-10h30 –  Developing antibodies for research, diagnostics and therapy using birds
11h00-12h00 – Visiting CNC’s Avian Facility

14h00- Mass Spectrometry
-Introduction to Mass Spectrometry and Liquid chromatography coupled to Mass spectrometry - Bruno Manadas
- Small molecule targeted analysis (case study of glucose, 2H and 13C glucose; drugs; metabolites and metabolomics) - Vera Mendes
- Introduction to LC-MS/MS based proteomics- Bruno Manadas
- Drug induced neuroproteomic changes - Cátia Santa
- Translational biomarker research - Sandra Anjo

Week II – 28th January – 1st February – CNC polo I

Monday, 28th January 2019
Fluorescence Microscopy
 9h30 - 10h30: Fundamentals of Fluorescence Microscopy – Luísa Cortes
11h00 - 12h00: Optical Sectioning – Luísa Cortes
14h00 - 15h30: Live Cell Imaging – Luísa Cortes
16h00 - 18h00: Hands on microscopy session I (*)

Tuesday, 29th January 2019

Flow Cytometry
10h00 - 11h00: Principals of flow cytometry – Isabel Nunes
11h15 - 12h00:  Flow Cytometry applications – Isabel Nunes

Fluorescence Microscopy
14h30 - 17h00: Hands on microscopy session II (*)

(*) Students will be divided in 2 groups for the hands-on sessions.

Wednesday, 30th January 2019
Fluorescence Microscopy
10h00 - 12h00: Hands on microscopy session III (*)
14h30 - 15h30: Fundamentals of Imaging Processing with FIJI – Luísa Cortes
15h45 - 17h30: Hands on imaging processing session

Thursday, 31st January 2019 – UC-Biotech

Flow Cytometry
10h00 - 12h30: Fluorescence-activated cell sorting (FACS): principles and applications – Marta Pinto
14h00 – 17h00: Hands on cell sorting session – Susana Pedreiro

Friday, 1st February 2019
15h00 - 16h00: Students Presentations

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